Category: Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Feb 15 2022

Magnetic particles for integrated nucleic acid purification, Quality Improvement of Capsular Polysaccharide in Streptococcus pneumoniae by Purification Process Optimization., Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Efficiency of a Single well IgG, IgM and IgA Anti T. gondii Fluorimetric Assay for Pre-natal Screening for Congenital Toxoplasmosis

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Toxoplasmosis, worldwide protozoan disease, is usually benign, except when acute disease occurs in pregnant women, resulting in fetal infection with deaths or high morbidity after birth. Treatment blocks fetal infection...

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Feb 15 2022

amplification and detection without pipetting, Magnetic particles for integrated nucleic acid purification, Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Vitamin C suppresses toxicological effects in MO/MФ and IgM + B cells of Nile tilapia (Oreochromis niloticus) upon copper exposure

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Copper (Cu), as an essential micronutrient in human and animal metabolism, easily spreads and excessively accumulates in rearing water, which make it more susceptible to fish farms and threatens the...

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Feb 15 2022

amplification and detection without pipetting, Magnetic particles for integrated nucleic acid purification, Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Performance of Rapid Antigen Tests for COVID-19 Diagnosis: A Systematic Review and Meta-Analysis

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The identification of viral RNA using reverse transcription quantitative polymerase chain reaction (RT-qPCR) is the gold standard for identifying an infection caused by SARS-CoV-2. The limitations of RT-qPCR such as...

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Feb 15 2022

Magnetic particles for integrated nucleic acid purification, Quality Improvement of Capsular Polysaccharide in Streptococcus pneumoniae by Purification Process Optimization., Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

An observational study to compare the diagnostic accuracy of rapid antigen test and rapid antibody tests in various periods of enteric fever

by Lieven

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A cross-sectional observational study was done on 180 fever patients to assess the diagnostic accuracy of rapid serological tests in early detection (<5 days of fever) of S. typhi and...

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Feb 15 2022

Magnetic particles for integrated nucleic acid purification, Quality Improvement of Capsular Polysaccharide in Streptococcus pneumoniae by Purification Process Optimization., Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance

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Post-load column wash in Protein A chromatography can effectively improve host cell protein (HCP) clearance. A commonly used wash additive for this purpose is sodium chloride. However, the adequate amount...

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Feb 15 2022

Magnetic particles for integrated nucleic acid purification, Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Chromatofocusing using micropellicular column packings with computer-aided design of the elution buffer composition

by Lieven

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Micropellicular, anion-exchange column packings are used in chromatofocusing to demonstrate the resolution and speed achieved when proteins are separated under these conditions. Linear or concave pH gradients are produced with...

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Feb 15 2022

Magnetic particles for integrated nucleic acid purification, Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Phi 6 Recovery from Inoculated Fingerpads Based on Elution Buffer and Methodology

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Phi 6 (Φ6) bacteriophage is a proposed surrogate to study pathogenic enveloped viruses including SARS-CoV-2-the causative agent of COVID-19-based on structural similarities, BSL-1 status, and ease of use. To determine...

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Feb 05 2022

Magnetic particles for integrated nucleic acid purification, Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

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CRISPR-Cas12a system has been shown promising for nucleic acid diagnostics due to its rapid, portable and accurate features. However, cleavage of the amplicons and primers by the cis– and trans-activity of Cas12a...

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May 29 2020

Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

by Lieven

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Oligodeoxynucleotides incorporating internucleotide phosphoroselenolate linkages have been ready beneath solid-phase synthesis circumstances utilizing dimer phosphoramidites. These dimers have been constructed following the excessive yielding Michaelis-Arbuzov (M-A) response of nucleoside H-phosphonate derivatives...

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