Oligodeoxynucleotides incorporating internucleotide phosphoroselenolate linkages have been ready beneath solid-phase synthesis circumstances utilizing dimer phosphoramidites. These dimers have been constructed following the excessive yielding Michaelis-Arbuzov (M-A) response of nucleoside H-phosphonate derivatives with 5′-deoxythymidine-5′-selenocyanate and subsequent phosphitylation.

Efficient coupling of the dimer phosphoramidites to solid-supported substrates was noticed beneath each handbook and automated circumstances and required solely minor modifications to the usual DNA synthesis cycle. In a additional demonstration of the utility of M-A chemistry, the support-bound selenonucleoside was reacted with an H-phosphonate and then chain prolonged utilizing phosphoramidite chemistry.

Following preliminary unmasking of methyl-protected phosphoroselenolate diesters, pure oligodeoxynucleotides have been remoted utilizing normal deprotection and purification procedures and subsequently characterised by mass spectrometry and round dichroism.

The CD spectra of each modified and native duplexes derived from self-complementary sequences with A-form, B-form or combined conformational preferences have been primarily superimposable. These sequences have been additionally used to check the impact of the modification upon duplex stability which confirmed context-dependent destabilisation (-0.four to -3.1 °C per phosphoroselenolate) when launched on the 5′-termini of A-form or combined duplexes or at juxtaposed central loci inside a B-form duplex (-1.0 °C per modification).

As discovered with different nucleic acids incorporating selenium, expeditious crystallisation of a modified decanucleotide A-form duplex was noticed and the construction solved to a decision of 1.45 Å. The DNA construction adjoining to the modification was not considerably perturbed. The phosphoroselenolate linkage was discovered to impart resistance to nuclease exercise.

Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.

A novel strategy for high-level expression and purification of GST-fused extremely thermostable Taq DNA polymerase in Escherichia coli.

Polymerases are enzymes that synthesize lengthy chains or polymers of nucleic acids together with DNA or RNA from nucleotides. They assemble nucleic acids by copying a DNA or RNA template strand utilizing base-pairing interactions. One of the polymerase enzymes, Taq DNA polymerase, initially remoted from Thermus aquaticus (Taq) is a extensively used enzyme in molecular biology up to now.

Mitochondria
MBS6507966-01mL 0.1mL
EUR 820

Mitochondria
MBS6507966-5x01mL 5x0.1mL
EUR 3530

Mitochondria
MBS6507967-01mL 0.1mL
EUR 765

Mitochondria
MBS6507967-5x01mL 5x0.1mL
EUR 3290

Mitochondria (AP)
MBS6124366-01mL 0.1(mL
EUR 935

Mitochondria (AP)
MBS6124366-5x01mL 5x0.1mL
EUR 4050

Mitochondria (PE)
MBS6124371-01mL 0.1(mL
EUR 935

Mitochondria (PE)
MBS6124371-5x01mL 5x0.1mL
EUR 4050

Mitochondria (AP)
MBS6123461-01mL 0.1(mL
EUR 935

Mitochondria (AP)
MBS6123461-5x01mL 5x0.1mL
EUR 4050

Mitochondria (PE)
MBS6123466-01mL 0.1(mL
EUR 935

Mitochondria (PE)
MBS6123466-5x01mL 5x0.1mL
EUR 4050

Mitochondria (AP)
MBS6123467-01mL 0.1(mL
EUR 935

Mitochondria (AP)
MBS6123467-5x01mL 5x0.1mL
EUR 4050

Mitochondria (PE)
MBS6123472-01mL 0.1(mL
EUR 935

Mitochondria (PE)
MBS6123472-5x01mL 5x0.1mL
EUR 4050

Mitochondria (AP)
MBS6123473-01mL 0.1(mL
EUR 935

Mitochondria (AP)
MBS6123473-5x01mL 5x0.1mL
EUR 4050

Mitochondria (PE)
MBS6123478-01mL 0.1(mL
EUR 935

Mitochondria (PE)
MBS6123478-5x01mL 5x0.1mL
EUR 4050

Mitochondria (AP)
MBS6123479-01mL 0.1(mL
EUR 935

Mitochondria (AP)
MBS6123479-5x01mL 5x0.1mL
EUR 4050

Mitochondria (PE)
MBS6123484-01mL 0.1(mL
EUR 935

Mitochondria (PE)
MBS6123484-5x01mL 5x0.1mL
EUR 4050

Mitochondria (AP)
MBS6241746-01mL 0.1(mL
EUR 1055

Mitochondria (AP)
MBS6241746-5x01mL 5x0.1mL
EUR 4595

Mitochondria (PE)
MBS6244410-01mL 0.1(mL
EUR 1055

Mitochondria (PE)
MBS6244410-5x01mL 5x0.1mL
EUR 4595

Mitochondria (APC)
MBS6124367-01mL 0.1(mL
EUR 935

Mitochondria (APC)
MBS6124367-5x01mL 5x0.1mL
EUR 4050

Mitochondria (HRP)
MBS6124370-01mL 0.1(mL
EUR 935

Mitochondria (HRP)
MBS6124370-5x01mL 5x0.1mL
EUR 4050

Mitochondria (APC)
MBS6123462-01mL 0.1(mL
EUR 935

Mitochondria (APC)
MBS6123462-5x01mL 5x0.1mL
EUR 4050

Mitochondria (HRP)
MBS6123465-01mL 0.1(mL
EUR 935

Mitochondria (HRP)
MBS6123465-5x01mL 5x0.1mL
EUR 4050

Mitochondria (APC)
MBS6123468-01mL 0.1(mL
EUR 935

Mitochondria (APC)
MBS6123468-5x01mL 5x0.1mL
EUR 4050

Mitochondria (HRP)
MBS6123471-01mL 0.1(mL
EUR 935

Mitochondria (HRP)
MBS6123471-5x01mL 5x0.1mL
EUR 4050

Mitochondria (APC)
MBS6123474-01mL 0.1(mL
EUR 935

Mitochondria (APC)
MBS6123474-5x01mL 5x0.1mL
EUR 4050

Mitochondria (HRP)
MBS6123477-01mL 0.1(mL
EUR 935

Mitochondria (HRP)
MBS6123477-5x01mL 5x0.1mL
EUR 4050

Mitochondria (APC)
MBS6123480-01mL 0.1(mL
EUR 935

Mitochondria (APC)
MBS6123480-5x01mL 5x0.1mL
EUR 4050

Mitochondria (HRP)
MBS6123483-01mL 0.1(mL
EUR 935

Mitochondria (HRP)
MBS6123483-5x01mL 5x0.1mL
EUR 4050

Mitochondria (HRP)
MBS6243877-01mL 0.1(mL
EUR 1055

Mitochondria (HRP)
MBS6243877-5x01mL 5x0.1mL
EUR 4595

Mitochondria (APC)
MBS6242279-01mL 0.1(mL
EUR 1055

Mitochondria (APC)
MBS6242279-5x01mL 5x0.1mL
EUR 4595

Mitochondria (FITC)
MBS6124369-01mL 0.1(mL
EUR 935

Mitochondria (FITC)
MBS6124369-5x01mL 5x0.1mL
EUR 4050

Mitochondria (FITC)
MBS6123464-01mL 0.1(mL
EUR 935

Mitochondria (FITC)
MBS6123464-5x01mL 5x0.1mL
EUR 4050

Mitochondria (FITC)
MBS6123470-01mL 0.1(mL
EUR 935

Mitochondria (FITC)
MBS6123470-5x01mL 5x0.1mL
EUR 4050

Mitochondria (FITC)
MBS6123476-01mL 0.1(mL
EUR 935

Mitochondria (FITC)
MBS6123476-5x01mL 5x0.1mL
EUR 4050

Mitochondria (FITC)
MBS6123482-01mL 0.1(mL
EUR 935

Mitochondria (FITC)
MBS6123482-5x01mL 5x0.1mL
EUR 4050

Mitochondria (FITC)
MBS6243344-01mL 0.1(mL
EUR 1055

Mitochondria (FITC)
MBS6243344-5x01mL 5x0.1mL
EUR 4595

Mitochondria (Biotin)
MBS6124368-01mL 0.1(mL
EUR 935

Mitochondria (Biotin)
MBS6124368-5x01mL 5x0.1mL
EUR 4050

Mitochondria (Biotin)
MBS6123463-01mL 0.1(mL
EUR 935

Mitochondria (Biotin)
MBS6123463-5x01mL 5x0.1mL
EUR 4050

Mitochondria (Biotin)
MBS6123469-01mL 0.1(mL
EUR 935

Mitochondria (Biotin)
MBS6123469-5x01mL 5x0.1mL
EUR 4050

Mitochondria (Biotin)
MBS6123475-01mL 0.1(mL
EUR 935

Mitochondria (Biotin)
MBS6123475-5x01mL 5x0.1mL
EUR 4050

Mitochondria (Biotin)
MBS6123481-01mL 0.1(mL
EUR 935

Mitochondria (Biotin)
MBS6123481-5x01mL 5x0.1mL
EUR 4050

Mitochondria (Biotin)
MBS6242811-01mL 0.1(mL
EUR 1055

Mitochondria (Biotin)
MBS6242811-5x01mL 5x0.1mL
EUR 4595

Mitochondria antibody
10R-8363 100 ul
EUR 451
Description: Mouse monoclonal Mitochondria antibody

Mitochondria (MaxLight 405)
MBS6244943-01mL 0.1(mL
EUR 1055

Mitochondria (MaxLight 405)
MBS6244943-5x01mL 5x0.1mL
EUR 4595

Mitochondria (MaxLight 490)
MBS6245476-01mL 0.1(mL
EUR 1055

Mitochondria (MaxLight 490)
MBS6245476-5x01mL 5x0.1mL
EUR 4595

Mitochondria (MaxLight 750)
MBS6247075-01mL 0.1(mL
EUR 1055

Mitochondria (MaxLight 750)
MBS6247075-5x01mL 5x0.1mL
EUR 4595

Mitochondria (MaxLight 550)
MBS6246009-01mL 0.1(mL
EUR 1055

Mitochondria (MaxLight 550)
MBS6246009-5x01mL 5x0.1mL
EUR 4595

Mitochondria (MaxLight 650)
MBS6246542-01mL 0.1(mL
EUR 1055

Mitochondria (MaxLight 650)
MBS6246542-5x01mL 5x0.1mL
EUR 4595

Mitochondria degrader-1
HY-147108 10 mg
EUR 1948.08
Description: Mitochondria degrader-1 (example 5) is a potent mitochondria degrader. Mitochondria degrader-1 induces the degradation of the injured mitochondria by the autophagy mechanism. Mitochondria degrader-1 can be used for the research of neurodegenerative disease, cancer, inflammatory disease, age-related disease, metabolic disease, mitochondrial disease or Down's disease[1].

Mitochondria Isolation Kit
AR0156 1 kit
EUR 127.2

Mitochondria Isolation Kit
BC156-50T 50T
EUR 150

Mitochondria Storage Buffer
M1104-25 each
EUR 288

Nano-Lantern Mitochondria
LVP1535 1x10^8 IFU/ml x 200ul
EUR 486.5
Description: Pre-made Nano-Lantern lentivirus for in vivo Luminescence Imaging of Mitochondria

Mitochondria Fractionation Kit
55R-1306 25 assays
EUR 567
Description: Mitochondria Fractionation Kit for use in the research laboratory

Mitochondria (BSA & Azide Free)
MBS6507968-01mg 0.1mg
EUR 805

Mitochondria (BSA & Azide Free)
MBS6507968-5x01mg 5x0.1mg
EUR 3470

Mitochondria (BSA & Azide Free)
MBS6268059-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free)
MBS6268059-5x01mL 5x0.1mL
EUR 4735

Cell Mitochondria Isolation Kit
abx090630-50100tests 50-100 tests
EUR 309.6

Cell Mitochondria Isolation Kit
ETF3601 100 assays
EUR 135

Mitochondria Activity Assay Kit
KC310100 1 kit
EUR 417

Cell Mitochondria Isolation Kit
abx090630-100l 100 µl
EUR 187.5

Cell Mitochondria Isolation Kit
abx090630-1ml 1 ml Ask for price

Cell Mitochondria Isolation Kit
abx090630-200l 200 µl Ask for price

Cell Mitochondria Isolation Kit
MBS8579523-100Assays 100Assays
EUR 245

Cell Mitochondria Isolation Kit
MBS8579523-5x100Assays 5x100Assays
EUR 1105

Cell Mitochondria Isolation Kit
MBS355486-50Tests 50Tests
EUR 255

Cell Mitochondria Isolation Kit
MBS355486-5x50Tests 5x50Tests
EUR 1130

Yeast Mitochondria (S. cerevisiae)
1222-200 each
EUR 510

Yeast Mitochondria (S. cerevisiae)
1222-50 each
EUR 248.4

Yeast Mitochondria Isolation Kit
K259-50 each
EUR 502.8

Tissue Mitochondria Isolation Kit
abx090631-50100assays 50-100 assays
EUR 309.6

Tissue Mitochondria Isolation Kit
ETF3606 100 assays
EUR 135

Tissue Mitochondria Isolation Kit
abx090631-100l 100 µl
EUR 187.5

Tissue Mitochondria Isolation Kit
abx090631-1ml 1 ml Ask for price

Tissue Mitochondria Isolation Kit
abx090631-200l 200 µl Ask for price

Tissue Mitochondria Isolation Kit
MBS8579524-100Assays 100Assays
EUR 245

Tissue Mitochondria Isolation Kit
MBS8579524-5x100Assays 5x100Assays
EUR 1105

Tissue Mitochondria Isolation Kit
MBS355483-50Tests 50Tests
EUR 255

Tissue Mitochondria Isolation Kit
MBS355483-5x50Tests 5x50Tests
EUR 1130

Mitochondria (BSA & Azide Free) (AP)
MBS6265817-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free) (AP)
MBS6265817-5x01mL 5x0.1mL
EUR 4735

Mitochondria (BSA & Azide Free) (PE)
MBS6266938-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free) (PE)
MBS6266938-5x01mL 5x0.1mL
EUR 4735

Yeast Mitochondria (Pichia pastoris)
1111-200 200 ug
EUR 354.24
Description: Mitochondria are the power house of the cells as they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles comprising of an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein profiling, and as a loading control. Highly pure yeast mitochondria are purified using standard protocol as mentioned in the Yeast Mitochondria Isolation Kit - Cat # K259-100 from Pichia pastoris.

Yeast Mitochondria (Pichia pastoris)
1111-50 50 ug
EUR 174.24
Description: Mitochondria are the power house of the cells as they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles comprising of an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein profiling, and as a loading control. Highly pure yeast mitochondria are purified using standard protocol as mentioned in the Yeast Mitochondria Isolation Kit - Cat # K259-100 from Pichia pastoris.

Alleleustrious pmTFP1-Mitochondria fusion vector Mitochondria
ABP-FP-TMIT 5 ug Ask for price

Alleleustrious pWasabi-Mitochondria fusion vector Mitochondria
ABP-FP-WMIT 5 ug Ask for price

Mitochondria (BSA & Azide Free) (HRP)
MBS6266713-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free) (HRP)
MBS6266713-5x01mL 5x0.1mL
EUR 4735

Mitochondria (BSA & Azide Free) (APC)
MBS6266041-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free) (APC)
MBS6266041-5x01mL 5x0.1mL
EUR 4735

Mitochondria (BSA & Azide Free) (FITC)
MBS6266489-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free) (FITC)
MBS6266489-5x01mL 5x0.1mL
EUR 4735

Mitochondria/Cytosol Fractionation Kit
K2103-100 100 assays
EUR 514
Description: Isolates mitochondria/cytosol w/o ultracentrifugation

Mitochondria/Cytosol Fractionation Kit
K2103-25 25 assays
EUR 274
Description: Isolates mitochondria/cytosol w/o ultracentrifugation

Mitochondria/Cytosol Fractionation Kit
KT-148 100 tests
EUR 544

OALA06680-50UL - Mitochondria Antibody
OALA06680-50UL 50ul
EUR 539

Mitochondria/Cytosol Fractionation Kit
K256-100 each
EUR 614.4

Mitochondria/Cytosol Fractionation Kit
K256-25 each
EUR 288

Mouse monoclonal Mitochondria antibody
MBS5306772-01mL 0.1mL
EUR 740

Mouse monoclonal Mitochondria antibody
MBS5306772-5x01mL 5x0.1mL
EUR 3170

Mitochondria (BSA & Azide Free) (Biotin)
MBS6266265-01mL 0.1mL
EUR 1085

Mitochondria (BSA & Azide Free) (Biotin)
MBS6266265-5x01mL 5x0.1mL
EUR 4735

Mito-Tracker Green (mitochondria probe)
ETF1048 50ug
EUR 100

Mito-Tracker Green (mitochondria probe)
MBS8579518-005mg 0.05mg
EUR 190

Mito-Tracker Green (mitochondria probe)
MBS8579518-5x005mg 5x0.05mg
EUR 710

COH-SR4 (Mitochondria uncoupler SR4)
MBS5759508-100mg 100mg
EUR 180

COH-SR4 (Mitochondria uncoupler SR4)
MBS5759508-200mg 200mg
EUR 220

COH-SR4 (Mitochondria uncoupler SR4)
MBS5759508-25mg 25mg
EUR 145

COH-SR4 (Mitochondria uncoupler SR4)
MBS5759508-50mg 50mg
EUR 150

COH-SR4 (Mitochondria uncoupler SR4)
MBS5759508-5x200mg 5x200mg
EUR 835

Mitochondria Labeling Lentivirus (MTS)(GFP)
LVP721 2 x 100ul , Titer: 10^9 IU/ml
EUR 895
Description: N/A

Mitochondria Labeling Lentivirus (MTS)(RFP)
LVP722 2 x 100ul , Titer: 10^9 IU/ml
EUR 895
Description: N/A

Mitochondria Labeling Lentivirus (MTS)(CFP)
LVP723 2 x 100ul , Titer: 10^9 IU/ml
EUR 895
Description: N/A

Mitochondria Isolation Kit (Cultured Cells)
K1138-10 10rxns
EUR 28
Description: Mitochondria Isolation Kit

The thermostable properties of this enzyme have contributed majorly to the specificity, automation, and efficacy of the polymerase chain response (PCR), making it a highly effective device for immediately’s molecular biology researches throughout the globe. The purification of Taq DNA polymerase from the native host ends in low yield, extra labor and time consumption.

Therefore, many research have been beforehand performed to acquire this enzyme utilizing different hosts. So far, all the present methodologies are extra laborious, time-consuming and require heavy expense. We used a novel strategy to purify the enzyme with comparatively excessive effectivity, yield and minimal time consumption utilizing Escherichia coli (E. coli) instead host.

We cloned a 2500 base pair Taq DNA polymerase gene into pGEX-4T-1 vector, containing a GST-tag, downstream of tac promoter and overexpressed it utilizing isopropyl β-d-1-thiogalactopyranoside (IPTG) as an inducer. The enzyme was effectively purified utilizing novel chromatography approaches and was utilized in routine PCR assays in our laboratory. Our findings counsel a novel strategy to facilitate the provision of polymerases for molecular and diagnostic research. In the longer term, it might be used for the purification of different recombinant peptides or proteins utilized in structural biology and proteomics-based researches.