Oligodeoxynucleotides incorporating internucleotide phosphoroselenolate linkages have been ready beneath solid-phase synthesis circumstances utilizing dimer phosphoramidites. These dimers have been constructed following the excessive yielding Michaelis-Arbuzov (M-A) response of nucleoside H-phosphonate derivatives with 5′-deoxythymidine-5′-selenocyanate and subsequent phosphitylation.
Efficient coupling of the dimer phosphoramidites to solid-supported substrates was noticed beneath each handbook and automated circumstances and required solely minor modifications to the usual DNA synthesis cycle. In a additional demonstration of the utility of M-A chemistry, the support-bound selenonucleoside was reacted with an H-phosphonate and then chain prolonged utilizing phosphoramidite chemistry.
Following preliminary unmasking of methyl-protected phosphoroselenolate diesters, pure oligodeoxynucleotides have been remoted utilizing normal deprotection and purification procedures and subsequently characterised by mass spectrometry and round dichroism.
The CD spectra of each modified and native duplexes derived from self-complementary sequences with A-form, B-form or combined conformational preferences have been primarily superimposable. These sequences have been additionally used to check the impact of the modification upon duplex stability which confirmed context-dependent destabilisation (-0.four to -3.1 °C per phosphoroselenolate) when launched on the 5′-termini of A-form or combined duplexes or at juxtaposed central loci inside a B-form duplex (-1.0 °C per modification).
As discovered with different nucleic acids incorporating selenium, expeditious crystallisation of a modified decanucleotide A-form duplex was noticed and the construction solved to a decision of 1.45 Å. The DNA construction adjoining to the modification was not considerably perturbed. The phosphoroselenolate linkage was discovered to impart resistance to nuclease exercise.

Solid-phase synthesis and structural characterisation of phosphoroselenolate-modified DNA: a backbone analogue which does not impose conformational bias and facilitates SAD X-ray crystallography.
A novel strategy for high-level expression and purification of GST-fused extremely thermostable Taq DNA polymerase in Escherichia coli.
Polymerases are enzymes that synthesize lengthy chains or polymers of nucleic acids together with DNA or RNA from nucleotides. They assemble nucleic acids by copying a DNA or RNA template strand utilizing base-pairing interactions. One of the polymerase enzymes, Taq DNA polymerase, initially remoted from Thermus aquaticus (Taq) is a extensively used enzyme in molecular biology up to now.
FCCP |
B5004-10 |
ApexBio |
10 mg |
EUR 132 |
FCCP |
B5004-50 |
ApexBio |
50 mg |
EUR 379 |
FCCP (MPTP inhibitor) |
SIH-222-10MG |
Stressmarq |
10 mg |
EUR 135 |
|
Description: The substance FCCP is a mptp inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is yellow solid which is soluble to 100 mM in DMSO. |
FCCP (MPTP inhibitor) |
SIH-222-50MG |
Stressmarq |
50 mg |
EUR 345 |
|
Description: The substance FCCP is a mptp inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is yellow solid which is soluble to 100 mM in DMSO. |
Alleleustrious pmTFP1-Mitochondria fusion vector Mitochondria |
ABP-FP-TMIT |
Allele Biotech |
5 ug |
Ask for price |
|
Alleleustrious pWasabi-Mitochondria fusion vector Mitochondria |
ABP-FP-WMIT |
Allele Biotech |
5 ug |
Ask for price |
|
Mitochondria antibody |
10R-8363 |
Fitzgerald |
100 ul |
EUR 349 |
Description: Mouse monoclonal Mitochondria antibody |
Mitochondria Isolation Kit |
AR0156 |
BosterBio |
1 kit |
EUR 106 |
Mitochondria Storage Buffer |
M1104-25 |
Biovision |
|
EUR 240 |
Mitochondria Fractionation Kit |
55R-1306 |
Fitzgerald |
25 assays |
EUR 296 |
Description: Mitochondria Fractionation Kit for use in the research laboratory |
Cell Mitochondria Isolation Kit |
abx090630-50100tests |
Abbexa |
50-100 tests |
EUR 258 |
|
Tissue Mitochondria Isolation Kit |
abx090631-50100assays |
Abbexa |
50-100 assays |
EUR 258 |
|
Mitochondria/Cytosol Fractionation Kit |
K2103-100 |
ApexBio |
100 assays |
EUR 529 |
Mitochondria/Cytosol Fractionation Kit |
K2103-25 |
ApexBio |
25 assays |
EUR 251 |
Mitochondria/Cytosol Fractionation Kit |
K256-100 |
Biovision |
|
EUR 512 |
Mitochondria/Cytosol Fractionation Kit |
K256-25 |
Biovision |
|
EUR 240 |
Yeast Mitochondria Isolation Kit |
K259-50 |
Biovision |
|
EUR 419 |
Yeast Mitochondria (Pichia pastoris) |
1111-200 |
Biovision |
200 ug |
EUR 295.2 |
|
Description: Mitochondria are the power house of the cells as they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles comprising of an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein profiling, and as a loading control. Highly pure yeast mitochondria are purified using standard protocol as mentioned in the Yeast Mitochondria Isolation Kit - Cat # K259-100 from Pichia pastoris. |
Yeast Mitochondria (Pichia pastoris) |
1111-50 |
Biovision |
50 ug |
EUR 145.2 |
|
Description: Mitochondria are the power house of the cells as they generate most of the supply of energy in the form of adenosine tri-phosphate (ATP). Mitochondria are double membrane organelles comprising of an outer membrane and a folded inner membrane called cristae. Isolated mitochondria are a useful tool to study mitochondrial respiration, assembly of the respiratory complexes, apoptosis, mtDNA, mtRNA, protein profiling, and as a loading control. Highly pure yeast mitochondria are purified using standard protocol as mentioned in the Yeast Mitochondria Isolation Kit - Cat # K259-100 from Pichia pastoris. |
Yeast Mitochondria (S. cerevisiae) |
1222-200 |
Biovision |
|
EUR 425 |
Yeast Mitochondria (S. cerevisiae) |
1222-50 |
Biovision |
|
EUR 207 |
Mitochondria Activity Assay Kit |
KC310100 |
Biochain |
1 kit |
EUR 398 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Mitochondria-eating protein (SPATA18) Antibody |
20-abx339523 |
Abbexa |
|
|
|
Mitochondria-eating protein (SPATA18) Antibody |
20-abx211393 |
Abbexa |
|
|
|
Recombinant human
Mitochondria-eating protein |
P1204 |
FN Test |
100ug |
Ask for price |
|
Description: Recombinant protein for human Mitochondria-eating protein |
Clustered Mitochondria Protein Homolog (CLUH) Antibody |
20-abx136050 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Mammalian Mitochondria Isolation Kit for Tissue |
abx098857-50rxns |
Abbexa |
50 rxns |
EUR 411 |
|
ATP Synthase gamma Chain, Mitochondria Protein |
20-abx261084 |
Abbexa |
-
EUR 3418.00
-
EUR 328.00
-
EUR 230.00
|
|
|
Mitochondria Isolation Kit (Mammalian cells / tissues) |
P507L |
101Bio |
- |
Ask for price |
Human Monofunctional C1- tetrahydrofolate synthase, mitochondria |
ELI-24270h |
Lifescience Market |
96 Tests |
EUR 824 |
Mouse Monofunctional C1- tetrahydrofolate synthase, mitochondria |
ELI-24946m |
Lifescience Market |
96 Tests |
EUR 865 |
Human Anti- mitochondria autoantibodies ELISA Kit |
ELA-E0354h |
Lifescience Market |
96 Tests |
EUR 824 |
Human M2 type mitochondria Antibody ELISA Kit |
DEIA1224 |
Creative Diagnostics |
96T |
EUR 944 |
Description: The Human M2 type mitochondria Antibody ELISA kit is designed to detect and quantify the level of human M2 type mitochondria Antibody in cell culture supernatant, serum, plasma and tissue. |
Human Anti-mitochondria autoantibodies(AMA)ELISA Kit |
GA-E0537HM-48T |
GenAsia Biotech |
48T |
EUR 289 |
Human Anti-mitochondria autoantibodies(AMA)ELISA Kit |
GA-E0537HM-96T |
GenAsia Biotech |
96T |
EUR 466 |
Human Anti-mitochondria autoantibodies,AMA ELISA Kit |
201-12-0521 |
SunredBio |
96 tests |
EUR 440 |
|
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Mammalian Mitochondria Isolation Kit for Cultured Cells |
20-abx098856 |
Abbexa |
|
|
|
ATP Synthase Subunit Gamma, Mitochondria (ATP5F1C) Antibody |
20-abx014219 |
Abbexa |
-
EUR 314.00
-
EUR 98.00
-
EUR 398.00
-
EUR 495.00
|
- 100 ug
- 10 ug
- 200 ug
- 300 µg
|
|
Mouse Mitochondria-eating protein (SPATA18) ELISA Kit |
abx390624-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Rat Mitochondria-eating protein (SPATA18) ELISA Kit |
abx391994-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Human Mitochondria-eating protein (SPATA18) ELISA Kit |
abx385435-96tests |
Abbexa |
96 tests |
EUR 911 |
|
Bovine Mitochondria- eating protein, SPATA18 ELISA KIT |
ELI-36655b |
Lifescience Market |
96 Tests |
EUR 928 |
Human Mitochondria- eating protein, SPATA18 ELISA KIT |
ELI-37181h |
Lifescience Market |
96 Tests |
EUR 824 |
Mouse Mitochondria- eating protein, Spata18 ELISA KIT |
ELI-42908m |
Lifescience Market |
96 Tests |
EUR 865 |
Chicken Mitochondria- eating protein, SPATA18 ELISA KIT |
ELI-13034c |
Lifescience Market |
96 Tests |
EUR 928 |
Porcine Mitochondria- eating protein, SPATA18 ELISA KIT |
ELI-16294p |
Lifescience Market |
96 Tests |
EUR 928 |
Human Anti-mitochondria autoantibodies,AMA ELISA Kit |
CN-03827H1 |
ChemNorm |
96T |
EUR 447 |
Human Anti-mitochondria autoantibodies,AMA ELISA Kit |
CN-03827H2 |
ChemNorm |
48T |
EUR 296 |
pCT-Mito-GFP (pCMV, Mitochondria, COX8 Tag) |
CYTO102-PA-1 |
SBI |
10 ug |
EUR 684 |
|
pCT-Mito-GFP (pMSCV, Mitochondria, COX8 Tag) |
CYTO102-PB-1 |
SBI |
10 ug |
EUR 684 |
|
pCT-Mito-GFP (pCMV, Mitochondria, COX8 Tag) |
CYTO102-VA-1 |
SBI |
>2x10^6 IFUs |
EUR 781 |
|
pCT-Mito-GFP (pMSCV, Mitochondria, COX8 Tag) |
CYTO102-VB-1 |
SBI |
>2x10^6 IFUs |
EUR 781 |
|
GATM ELISA Kit| Bovine Glycine amidinotransferase, mitochondria |
EF011444 |
Lifescience Market |
96 Tests |
EUR 689 |
Anti-Mitochondria Antibody Clone SPM198, Unconjugated-100ug |
MSM1X-739-P1 |
EnQuireBio |
100ug |
EUR 428 |
Anti-Mitochondria Antibody Clone MTC02, Unconjugated-100ug |
MSM2-740-P1 |
EnQuireBio |
100ug |
EUR 428 |
Anti-Mitochondria Antibody Clone MTC719, Unconjugated-100ug |
MSM3-719-P1 |
EnQuireBio |
100ug |
EUR 428 |
Anti-Mitochondria Antibody Clone MTC754, Unconjugated-100ug |
MSM4-754-P1 |
EnQuireBio |
100ug |
EUR 428 |
Rat Anti mitochondria autoantibodies,AMA ELISA kit |
E02A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Anti mitochondria autoantibodies,AMA ELISA kit |
E02A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti mitochondria autoantibodies,AMA ELISA kit |
E08A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti mitochondria autoantibodies,AMA ELISA kit |
E08A2090-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Anti mitochondria autoantibodies,AMA ELISA kit |
E08A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Mitochondria eating protein(SPATA18) ELISA kit |
E07M0439-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Mitochondria eating protein(SPATA18) ELISA kit |
E07M0439-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Mitochondria eating protein(SPATA18) ELISA kit |
E07M0439-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Anti mitochondria autoantibodies,AMA ELISA kit |
E09A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Anti mitochondria autoantibodies,AMA ELISA kit |
E09A2090-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Monkey Anti mitochondria autoantibodies,AMA ELISA kit |
E09A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Monkey Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Mitochondria eating protein(SPATA18) ELISA kit |
E08M0439-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Mitochondria eating protein(SPATA18) ELISA kit |
E08M0439-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Mitochondria eating protein(SPATA18) ELISA kit |
E08M0439-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Canine Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Mitochondria eating protein(SPATA18) ELISA kit |
E04M0439-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Mitochondria eating protein(SPATA18) ELISA kit |
E04M0439-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Mitochondria eating protein(SPATA18) ELISA kit |
E04M0439-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Mitochondria eating protein(SPATA18) ELISA kit |
E06M0439-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Mitochondria eating protein(SPATA18) ELISA kit |
E06M0439-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Mitochondria eating protein(SPATA18) ELISA kit |
E06M0439-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti mitochondria autoantibodies,AMA ELISA kit |
E04A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti mitochondria autoantibodies,AMA ELISA kit |
E04A2090-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rabbit Anti mitochondria autoantibodies,AMA ELISA kit |
E04A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Rabbit Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti mitochondria autoantibodies,AMA ELISA kit |
E03A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti mitochondria autoantibodies,AMA ELISA kit |
E03A2090-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Anti mitochondria autoantibodies,AMA ELISA kit |
E03A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Mitochondria eating protein(SPATA18) ELISA kit |
E03M0439-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Mitochondria eating protein(SPATA18) ELISA kit |
E03M0439-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Mitochondria eating protein(SPATA18) ELISA kit |
E03M0439-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Mouse Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti mitochondria autoantibodies,AMA ELISA kit |
E07A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti mitochondria autoantibodies,AMA ELISA kit |
E07A2090-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Pig Anti mitochondria autoantibodies,AMA ELISA kit |
E07A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Porcine Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti mitochondria autoantibodies,AMA ELISA kit |
E06A2090-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti mitochondria autoantibodies,AMA ELISA kit |
E06A2090-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Goat Anti mitochondria autoantibodies,AMA ELISA kit |
E06A2090-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A sandwich ELISA for quantitative measurement of Goat Anti mitochondria autoantibodies,AMA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Mitochondria eating protein(SPATA18) ELISA kit |
E02M0439-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Mitochondria eating protein(SPATA18) ELISA kit |
E02M0439-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A sandwich ELISA for quantitative measurement of Rat Mitochondria eating protein(SPATA18) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
The thermostable properties of this enzyme have contributed majorly to the specificity, automation, and efficacy of the polymerase chain response (PCR), making it a highly effective device for immediately’s molecular biology researches throughout the globe. The purification of Taq DNA polymerase from the native host ends in low yield, extra labor and time consumption.
Therefore, many research have been beforehand performed to acquire this enzyme utilizing different hosts. So far, all the present methodologies are extra laborious, time-consuming and require heavy expense. We used a novel strategy to purify the enzyme with comparatively excessive effectivity, yield and minimal time consumption utilizing Escherichia coli (E. coli) instead host.
We cloned a 2500 base pair Taq DNA polymerase gene into pGEX-4T-1 vector, containing a GST-tag, downstream of tac promoter and overexpressed it utilizing isopropyl β-d-1-thiogalactopyranoside (IPTG) as an inducer. The enzyme was effectively purified utilizing novel chromatography approaches and was utilized in routine PCR assays in our laboratory. Our findings counsel a novel strategy to facilitate the provision of polymerases for molecular and diagnostic research. In the longer term, it might be used for the purification of different recombinant peptides or proteins utilized in structural biology and proteomics-based researches.