Parallel poly(A) homo- and hetero-duplex formation detection with an adapted DNA nanoswitch technique.

Polyriboadenylic (poly(rA)) strands of sufficient length form parallel double helices in acidic and/or ammonium-containing conditions. Poly(rA) duplexes in acidic conditions are held together by A⁺-A⁺ base-pairing also involving base interactions with the phosphate backbone.

Traditional UV-melting studies of parallel poly(A) duplexes have typically examined homo-duplex formation of a single nucleic acid species in solution. We have adapted a technique utilizing a DNA nanoswitch that detects interaction of two different strands either with similar or differing lengths or modifications.

SAM FCM (Alexa Fluor 488)
abx098904-60tests	Abbexa	60 tests	EUR 1629.6

FCMR
pro-2579	ProSpec Tany	2µg	EUR 60
Description: Recombinant Human Fc Fragment Of IgM Receptor

FCMR Antibody
1-CSB-PA007972ESR2HU	Cusabio	EUR 266.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against FCMR. Recognizes FCMR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Fcmr Antibody
1-CSB-PA007972LA01MO	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000

FCMR Antibody
E40PV7165	EnoGene	50ul	EUR 395
Description: Available in various conjugation types.

Fcmr Antibody
CAC10363-100ug	Biomatik Corporation	100ug	EUR 314

Fcmr Antibody
CAC10363-50ug	Biomatik Corporation	50ug	EUR 199.2

Human FCMR Protein, Fc Tag
E40KMPH4349	EnoGene	20ug	EUR 495

Human IgM protein (Fc5mu) (HRP)
65C-CH0918	Fitzgerald	1 mg	EUR 868.8
Description: Human IgM protein (Fc5mu) (HRP) conjugate

Human IgM protein (Fc5mu) (FITC)
65C-CH0917	Fitzgerald	1 mg	EUR 685.2
Description: Human IgM protein (Fc5mu) (FITC) conjugate

FCMTE1 ORF Vector (Human) (pORF)
ORF019527	ABM	1.0 ug DNA	Ask for price

FCMTE2 ORF Vector (Human) (pORF)
ORF019528	ABM	1.0 ug DNA	Ask for price

FCMTE3 ORF Vector (Human) (pORF)
ORF019529	ABM	1.0 ug DNA	Ask for price

FCMTE1 3'UTR GFP Stable Cell Line
TU057841	ABM	1.0 ml	EUR 2799.6

FCMTE2 3'UTR GFP Stable Cell Line
TU057842	ABM	1.0 ml	EUR 2799.6

FCMTE3 3'UTR GFP Stable Cell Line
TU057843	ABM	1.0 ml	EUR 2799.6

Fcmr Polyclonal Antibody
A56202	EpiGentek	EUR 684.66 Ask for price Ask for price EUR 423.50	100 µg 20 ul 50 ul 100 ul

Human FCMR Protein Lysate 20ug
IHUFCMRPLLY20UG	Innovative research	each	EUR 213
Description: Human FCMR Protein Lysate 20ug

Fcmr Antibody, HRP conjugated
1-CSB-PA007972LB01MO	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA

Fcmr Antibody, FITC conjugated
1-CSB-PA007972LC01MO	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA

Fcmr Antibody, Biotin conjugated
1-CSB-PA007972LD01MO	Cusabio	EUR 380.40 EUR 402.00	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA

FCMTE1 Protein Vector (Human) (pPM-C-HA)
PV078107	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPM-C-HA)
PV078111	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPM-C-HA)
PV078115	ABM	500 ng	Ask for price

Fc Fragment Of IgM Receptor (FCMR) Antibody
20-abx109287	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Fc Fragment of IgM Receptor (FCMR) Antibody
20-abx320226	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody
20-abx339450	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody
20-abx339609	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

FCMTE1 Protein Vector (Human) (pPB-C-His)
PV078105	ABM	500 ng	Ask for price

FCMTE1 Protein Vector (Human) (pPB-N-His)
PV078106	ABM	500 ng	Ask for price

FCMTE1 Protein Vector (Human) (pPM-C-His)
PV078108	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPB-C-His)
PV078109	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPB-N-His)
PV078110	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPM-C-His)
PV078112	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPB-C-His)
PV078113	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPB-N-His)
PV078114	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPM-C-His)
PV078116	ABM	500 ng	Ask for price

FCMTE1 Recombinant Protein (Human)
RP058578	ABM	100 ug	Ask for price

FCMTE2 Recombinant Protein (Human)
RP058581	ABM	100 ug	Ask for price

FCMTE3 Recombinant Protein (Human)
RP058584	ABM	100 ug	Ask for price

FCMTE1 3'UTR Luciferase Stable Cell Line
TU007841	ABM	1.0 ml	EUR 2799.6

FCMTE2 3'UTR Luciferase Stable Cell Line
TU007842	ABM	1.0 ml	EUR 2799.6

FCMTE3 3'UTR Luciferase Stable Cell Line
TU007843	ABM	1.0 ml	EUR 2799.6

Fc Fragment Of IgM Receptor (FCMR) Antibody (HRP)
20-abx108239	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Fc Fragment Of IgM Receptor (FCMR) Antibody (FITC)
20-abx106820	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Fc Fragment Of IgM Receptor (FCMR) Antibody (Biotin)
20-abx105401	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Fcmr Polyclonal Antibody, HRP Conjugated
A56203	EpiGentek	EUR 684.66 EUR 302.50 EUR 423.50	100 µg 50 ul 100 ul

Polyclonal FCMR / FAIM3 Antibody (C-Terminus)
APR02365G	Leading Biology	0.05mg	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FCMR / FAIM3 (C-Terminus). This antibody is tested and proven to work in the following applications:

Fcmr Polyclonal Antibody, FITC Conjugated
A56204	EpiGentek	EUR 684.66 EUR 302.50 EUR 423.50	100 µg 50 ul 100 ul

Fcmr Polyclonal Antibody, Biotin Conjugated
A56205	EpiGentek	EUR 684.66 EUR 302.50 EUR 423.50	100 µg 50 ul 100 ul

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV725231	ABM	1.0 ug DNA	Ask for price

FCMTE1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV725235	ABM	1.0 ug DNA	Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV725237	ABM	1.0 ug DNA	Ask for price

FCMTE2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV725241	ABM	1.0 ug DNA	Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV725243	ABM	1.0 ug DNA	Ask for price

FCMTE3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV725247	ABM	1.0 ug DNA	Ask for price

FCMTE1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV725236	ABM	1.0 ug DNA	Ask for price

FCMTE2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV725242	ABM	1.0 ug DNA	Ask for price

FCMTE3 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV725248	ABM	1.0 ug DNA	Ask for price

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV725232	ABM	1.0 ug DNA	Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV725238	ABM	1.0 ug DNA	Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV725244	ABM	1.0 ug DNA	Ask for price

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV725233	ABM	1.0 ug DNA	Ask for price

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV725234	ABM	1.0 ug DNA	Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV725239	ABM	1.0 ug DNA	Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV725240	ABM	1.0 ug DNA	Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV725245	ABM	1.0 ug DNA	Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV725246	ABM	1.0 ug DNA	Ask for price

Recombinant Mouse Fas apoptotic inhibitory molecule 3 (Fcmr) ,partial
RPC21299-100ug	Biomatik Corporation	100ug	EUR 705.1

Recombinant Mouse Fas apoptotic inhibitory molecule 3 (Fcmr) ,partial
RPC21299-1mg	Biomatik Corporation	1mg	EUR 3237

Recombinant Mouse Fas apoptotic inhibitory molecule 3 (Fcmr) ,partial
RPC21299-20ug	Biomatik Corporation	20ug	EUR 353.4

Fukuyama type congenital muscular dystrophy protein (FCMD) polyclonal antibody
ABP-PAB-10673	Allele Biotech	100 ug	Ask for price

Our method detected parallel duplex formation as a function of length, chemical modifications, and pH, and at a sensitivity that required over 100 fold less concentration of sample than prior UV-melting methods. While parallel polyriboadenylic acid and poly-2′-O-methyl-adenylic acid homo-duplexes formed, we did not detect homo-duplexes of polydeoxyriboadenylic acid strands or poly-locked nucleic acid (LNA)-adenylic strands.

Importantly however, a poly-locked nucleic acid (LNA)-adenylic strand, as well as a poly-2′-O-methyl-adenylic strand formed a hetero-duplex with a polyriboadenylic strand. Overall, our work validates a new tool for studying parallel duplexes and reveals fundamental properties of poly(A) parallel duplex formation. Parallel duplexes may find use in DNA nanotechnology and in molecular biology applications such as a potential poly(rA) tail capture tool as an alternative to traditional oligo(dT) based purification.