Parallel poly(A) homo- and hetero-duplex formation detection with an adapted DNA nanoswitch technique.

Polyriboadenylic (poly(rA)) strands of sufficient length form parallel double helices in acidic and/or ammonium-containing conditions. Poly(rA) duplexes in acidic conditions are held together by A⁺-A⁺ base-pairing also involving base interactions with the phosphate backbone.

Traditional UV-melting studies of parallel poly(A) duplexes have typically examined homo-duplex formation of a single nucleic acid species in solution. We have adapted a technique utilizing a DNA nanoswitch that detects interaction of two different strands either with similar or differing lengths or modifications.

SAM FCM (Alexa Fluor 488)
abx098904-60tests	Abbexa	60 tests	EUR 1629.6

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (AP)
MBS6448043-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (AP)
MBS6448043-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (PE)
MBS6448053-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (PE)
MBS6448053-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (APC)
MBS6448044-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (APC)
MBS6448044-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (FITC)
MBS6448046-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (FITC)
MBS6448046-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (HRP).
MBS6448047-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (HRP).
MBS6448047-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (Biotin)
MBS6448045-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (Biotin)
MBS6448045-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 405)
MBS6448048-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 405)
MBS6448048-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 490)
MBS6448049-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 490)
MBS6448049-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 550)
MBS6448050-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 550)
MBS6448050-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 650)
MBS6448051-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 650)
MBS6448051-5x01mL	MyBiosource	5x0.1mL	EUR 4120

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 750)
MBS6448052-01mL	MyBiosource	0.1mL	EUR 950

NOL3 (Nucleolar Protein 3, ARC, FCM, MYP, NOP, NOP30, Apoptosis repressor with CARD) (MaxLight 750)
MBS6448052-5x01mL	MyBiosource	5x0.1mL	EUR 4120

FCMR
pro-2579	ProSpec Tany	2µg	EUR 60
Description: Recombinant Human Fc Fragment Of IgM Receptor

FCMR Antibody
E40PV7165	EnoGene	50ul	EUR 395
Description: Available in various conjugation types.

Fcmr Antibody
CAC10363-100ug	Biomatik Corporation	100ug	EUR 314

Fcmr Antibody
CAC10363-50ug	Biomatik Corporation	50ug	EUR 199.2

FCMR Antibody
1-CSB-PA007972ESR2HU	Cusabio	Ask for price Ask for price	100ul 50ul
Description: A polyclonal antibody against FCMR. Recognizes FCMR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Fcmr Antibody
1-CSB-PA007972LA01MO	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000

Fc-mDectin-1a
fc-mdec1a-2	InvivoGen FR	2 x 50 µg	EUR 454.65

Human FCMR Protein, Fc Tag
E40KMPH4349	EnoGene	20ug	EUR 495

Human FCMR Protein, hFc Tag
MBS188367-001mg	MyBiosource	0.01mg	EUR 235

Human FCMR Protein, hFc Tag
MBS188367-005mg	MyBiosource	0.05mg	EUR 435

Human FCMR Protein, hFc Tag
MBS188367-5x005mg	MyBiosource	5x0.05mg	EUR 1655

Human IgM protein (Fc5mu) (HRP)
65C-CH0918	Fitzgerald	1 mg	EUR 787
Description: Human IgM protein (Fc5mu) (HRP) conjugate

Human IgM protein (Fc5mu) (HRP)
MBS538976-1mg	MyBiosource	1mg	EUR 1265

Human IgM protein (Fc5mu) (HRP)
MBS538976-5x1mg	MyBiosource	5x1mg	EUR 5535

Human IgM protein (Fc5mu) (FITC)
65C-CH0917	Fitzgerald	1 mg	EUR 598
Description: Human IgM protein (Fc5mu) (FITC) conjugate

FCMTE1 3'UTR GFP Stable Cell Line
TU057841	ABM	1.0 ml	EUR 2799.6

FCMTE2 3'UTR GFP Stable Cell Line
TU057842	ABM	1.0 ml	EUR 2799.6

FCMTE3 3'UTR GFP Stable Cell Line
TU057843	ABM	1.0 ml	EUR 2799.6

Anti-Toso FCMR Antibody
A09423	BosterBio	0.1mg	EUR 449
Description: Boster Bio Anti-Toso FCMR Antibody (Catalog # A09423). Tested in ELISA, WB applications. This antibody reacts with Human.

FCMTE1 ORF Vector (Human) (pORF)
ORF019527	ABM	1.0 ug DNA	Ask for price

FCMTE2 ORF Vector (Human) (pORF)
ORF019528	ABM	1.0 ug DNA	Ask for price

FCMTE3 ORF Vector (Human) (pORF)
ORF019529	ABM	1.0 ug DNA	Ask for price

Human FCMR Protein Lysate
MBS8411681-002mg	MyBiosource	0.02mg	EUR 365

Human FCMR Protein Lysate
MBS8411681-5x002mg	MyBiosource	5x0.02mg	EUR 1410

Human IgM protein (Fc5mu) (FITC)
MBS538975-1mg	MyBiosource	1mg	EUR 985

Human IgM protein (Fc5mu) (FITC)
MBS538975-5x1mg	MyBiosource	5x1mg	EUR 4270

FCMR Peptide - middle region
MBS3245281-01mg	MyBiosource	0.1mg	EUR 180

FCMR Peptide - middle region
MBS3245281-5x01mg	MyBiosource	5x0.1mg	EUR 730

Fcmr Polyclonal Antibody
A56202	EpiGentek	Ask for price Ask for price Ask for price Ask for price	100 µg 20 ul 50 ul 100 ul

Human FCMR Protein Lysate 20ug
IHUFCMRPLLY20UG	Innovative research	each	EUR 213
Description: Human FCMR Protein Lysate 20ug

FCMR Antibody - middle region
MBS3220480-01mL	MyBiosource	0.1mL	EUR 455

FCMR Antibody - middle region
MBS3220480-5x01mL	MyBiosource	5x0.1mL	EUR 1995

Fcmr Antibody, HRP conjugated
1-CSB-PA007972LB01MO	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA

Fcmr Antibody, FITC conjugated
1-CSB-PA007972LC01MO	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA

IgM Fc5mu fragment, Human, Myeloma
MBS6120666-1mg	MyBiosource	1(mg	EUR 730

IgM Fc5mu fragment, Human, Myeloma
MBS6120666-5x1mg	MyBiosource	5x1mg	EUR 3065

IgM Fc5mu fragment, Human, Myeloma
MBS654670-1mg	MyBiosource	1mg	EUR 730

IgM Fc5mu fragment, Human, Myeloma
MBS654670-5x1mg	MyBiosource	5x1mg	EUR 3130

OAAN01731-50UL - FCMR Antibody
OAAN01731-50UL	Aviva Systems Biology	50ul	EUR 149

OACA06922-50UL - FCMR Antibody
OACA06922-50UL	Aviva Systems Biology	50ul	EUR 189

OACA02945-50UG - FCMR Antibody
OACA02945-50UG	Aviva Systems Biology	50ug	EUR 179

IgM Fc5mu fragment, Human, Myeloma (HRP)
MBS654860-1mg	MyBiosource	1mg	EUR 985

IgM Fc5mu fragment, Human, Myeloma (HRP)
MBS654860-5x1mg	MyBiosource	5x1mg	EUR 4290

Fcmr Antibody, Biotin conjugated
1-CSB-PA007972LD01MO	Cusabio	Ask for price Ask for price	100ug 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA

OAAN01731-100UL - FCMR Antibody
OAAN01731-100UL	Aviva Systems Biology	100ul	EUR 249

OAAN01731-200UL - FCMR Antibody
OAAN01731-200UL	Aviva Systems Biology	200ul	EUR 389

OACA06922-100UL - FCMR Antibody
OACA06922-100UL	Aviva Systems Biology	100ul	EUR 230

OACA02945-100UG - FCMR Antibody
OACA02945-100UG	Aviva Systems Biology	100ug	EUR 319

FCMTE1 Protein Vector (Human) (pPM-C-HA)
PV078107	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPM-C-HA)
PV078111	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPM-C-HA)
PV078115	ABM	500 ng	Ask for price

FCMR Rabbit Polyclonal Antibody
E10G01349	EnoGene	100 μl	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

FCMR Rabbit Polyclonal Antibody
E10G08305	EnoGene	100 μl	EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

IgM Fc5mu fragment, Human, Myeloma (FITC)
MBS654806-1mg	MyBiosource	1mg	EUR 835

IgM Fc5mu fragment, Human, Myeloma (FITC)
MBS654806-5x1mg	MyBiosource	5x1mg	EUR 3610

Fc Fragment Of IgM Receptor (FCMR) Antibody
20-abx109287	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Fc Fragment of IgM Receptor (FCMR) Antibody
20-abx320226	Abbexa	Ask for price Ask for price	100 ul 50 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody
20-abx339450	Abbexa	Ask for price Ask for price	100 ul 50 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody
20-abx339609	Abbexa	Ask for price Ask for price	100 ul 50 ul

FCMTE1 3'UTR Luciferase Stable Cell Line
TU007841	ABM	1.0 ml	EUR 2799.6

FCMTE2 3'UTR Luciferase Stable Cell Line
TU007842	ABM	1.0 ml	EUR 2799.6

FCMTE3 3'UTR Luciferase Stable Cell Line
TU007843	ABM	1.0 ml	EUR 2799.6

FCMTE1 Protein Vector (Human) (pPB-C-His)
PV078105	ABM	500 ng	Ask for price

FCMTE1 Protein Vector (Human) (pPB-N-His)
PV078106	ABM	500 ng	Ask for price

FCMTE1 Protein Vector (Human) (pPM-C-His)
PV078108	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPB-C-His)
PV078109	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPB-N-His)
PV078110	ABM	500 ng	Ask for price

FCMTE2 Protein Vector (Human) (pPM-C-His)
PV078112	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPB-C-His)
PV078113	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPB-N-His)
PV078114	ABM	500 ng	Ask for price

FCMTE3 Protein Vector (Human) (pPM-C-His)
PV078116	ABM	500 ng	Ask for price

FCMTE1 Recombinant Protein (Human)
RP058578	ABM	100 ug	Ask for price

FCMTE2 Recombinant Protein (Human)
RP058581	ABM	100 ug	Ask for price

FCMTE3 Recombinant Protein (Human)
RP058584	ABM	100 ug	Ask for price

Fc Fragment Of IgM Receptor (FCMR) Antibody (HRP)
20-abx108239	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

OCOA21346-20UG - FCMR Protein Lysate
OCOA21346-20UG	Aviva Systems Biology	20ug	EUR 169

OCOA01524-20UG - FCMR Protein Lysate
OCOA01524-20UG	Aviva Systems Biology	20ug	EUR 169

FAIM3 (FCMR) (NM_001142472) Human Recombinant Protein
PROTO60667	BosterBio	20ug	EUR 1249
Description: Purified recombinant protein of Homo sapiens Fas apoptotic inhibitory molecule 3 (FAIM3 /Toso), transcript variant 2, residue Arg18 -Gly251, expressed in HEK293 cells, with cterminal Fc tag

Fc Fragment Of IgM Receptor (FCMR) Antibody (FITC)
20-abx106820	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

FAIM3 (FCMR) (NM_005449) Human Over-expression Lysate
LS009214	BosterBio	100ug	EUR 628
Description: Transient overexpression lysate of Fas apoptotic inhibitory molecule 3 (FAIM3), transcript variant 1

FCMR/FAIM3 Protein, Human, Recombinant (hFc Tag)
MBS8121465-01mg	MyBiosource	0.1mg	EUR 395

FCMR/FAIM3 Protein, Human, Recombinant (hFc Tag)
MBS8121465-5x01mg	MyBiosource	5x0.1mg	EUR 1635

Fc Fragment Of IgM Receptor (FCMR) Antibody (Biotin)
20-abx105401	Abbexa	Ask for price Ask for price Ask for price Ask for price Ask for price	100 ug 1 mg 200 ug 20 ug 50 ug

Fcmr Polyclonal Antibody, HRP Conjugated
A56203	EpiGentek	Ask for price Ask for price Ask for price	100 µg 50 ul 100 ul

Fcmr (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
SR413999	Origene Technologies GmbH	2 nmol	Ask for price

FCMR (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
SR322699	Origene Technologies GmbH	2 nmol	Ask for price

Polyclonal FCMR / FAIM3 Antibody (C-Terminus)
APR02365G	Leading Biology	0.05mg	EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FCMR / FAIM3 (C-Terminus). This antibody is tested and proven to work in the following applications:

Fcmr Polyclonal Antibody, FITC Conjugated
A56204	EpiGentek	Ask for price Ask for price Ask for price	100 µg 50 ul 100 ul

ARP76846_P050-25UL - FCMR Antibody - middle region
ARP76846_P050-25UL	Aviva Systems Biology	25ul	EUR 99

Fcmr Polyclonal Antibody, Biotin Conjugated
A56205	EpiGentek	Ask for price Ask for price Ask for price	100 µg 50 ul 100 ul

Human FCMR/FAIM3 HEK293 Overexpression Lysate
MBS8115207-03mg	MyBiosource	0.3mg	EUR 280

Human FCMR/FAIM3 HEK293 Overexpression Lysate
MBS8115207-5x03mg	MyBiosource	5x0.3mg	EUR 1205

FAIM3 (FCMR) rabbit polyclonal antibody, Purified
AP07299PU-N	Origene Technologies GmbH	50 µg	Ask for price

Rat Fcmr / Fas apoptotic inhibitory molecule 3 ELISA Kit
E15361r	EIAab	96T	EUR 796

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (AP)
MBS6446459-01mL	MyBiosource	0.1mL	EUR 950

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (AP)
MBS6446459-5x01mL	MyBiosource	5x0.1mL	EUR 4120

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (PE)
MBS6446469-01mL	MyBiosource	0.1mL	EUR 950

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (PE)
MBS6446469-5x01mL	MyBiosource	5x0.1mL	EUR 4120

Rat Fcmr/Fas Apoptotic Inhibitory Molecule 3 ELISA Kit
MBS2890765-10x96StripWells	MyBiosource	10x96-Strip-Wells	EUR 4080

Rat Fcmr/Fas Apoptotic Inhibitory Molecule 3 ELISA Kit
MBS2890765-48StripWells	MyBiosource	48-Strip-Wells	EUR 390

Rat Fcmr/Fas Apoptotic Inhibitory Molecule 3 ELISA Kit
MBS2890765-5x96StripWells	MyBiosource	5x96-Strip-Wells	EUR 2220

Rat Fcmr/Fas Apoptotic Inhibitory Molecule 3 ELISA Kit
MBS2890765-96StripWells	MyBiosource	96-Strip-Wells	EUR 520

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (APC)
MBS6446460-01mL	MyBiosource	0.1mL	EUR 950

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (APC)
MBS6446460-5x01mL	MyBiosource	5x0.1mL	EUR 4120

FAIM3 (FCMR, TOSO, Fas Apoptotic Inhibitory Molecule 3) (HRP).
MBS6446463-01mL	MyBiosource	0.1mL	EUR 950

Our method detected parallel duplex formation as a function of length, chemical modifications, and pH, and at a sensitivity that required over 100 fold less concentration of sample than prior UV-melting methods. While parallel polyriboadenylic acid and poly-2′-O-methyl-adenylic acid homo-duplexes formed, we did not detect homo-duplexes of polydeoxyriboadenylic acid strands or poly-locked nucleic acid (LNA)-adenylic strands.

Importantly however, a poly-locked nucleic acid (LNA)-adenylic strand, as well as a poly-2′-O-methyl-adenylic strand formed a hetero-duplex with a polyriboadenylic strand. Overall, our work validates a new tool for studying parallel duplexes and reveals fundamental properties of poly(A) parallel duplex formation. Parallel duplexes may find use in DNA nanotechnology and in molecular biology applications such as a potential poly(rA) tail capture tool as an alternative to traditional oligo(dT) based purification.