Parallel poly(A) homo- and hetero-duplex formation detection with an adapted DNA nanoswitch technique.

Polyriboadenylic (poly(rA)) strands of sufficient length form parallel double helices in acidic and/or ammonium-containing conditions. Poly(rA) duplexes in acidic conditions are held together by A+-A+ base-pairing also involving base interactions with the phosphate backbone.

Traditional UV-melting studies of parallel poly(A) duplexes have typically examined homo-duplex formation of a single nucleic acid species in solution. We have adapted a technique utilizing a DNA nanoswitch that detects interaction of two different strands either with similar or differing lengths or modifications.

SAM FCM (Alexa Fluor 488)

abx098904-60tests 60 tests
EUR 1629.6

FCMR Antibody

1-CSB-PA007972ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against FCMR. Recognizes FCMR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

Fcmr Antibody

1-CSB-PA007972LA01MO
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000

Human IgM protein (Fc5mu) (HRP)

65C-CH0918 1 mg
EUR 868.8
Description: Human IgM protein (Fc5mu) (HRP) conjugate

Human IgM protein (Fc5mu) (FITC)

65C-CH0917 1 mg
EUR 685.2
Description: Human IgM protein (Fc5mu) (FITC) conjugate

FCMTE1 ORF Vector (Human) (pORF)

ORF019527 1.0 ug DNA Ask for price

FCMTE2 ORF Vector (Human) (pORF)

ORF019528 1.0 ug DNA Ask for price

FCMTE3 ORF Vector (Human) (pORF)

ORF019529 1.0 ug DNA Ask for price

FCMTE1 3'UTR GFP Stable Cell Line

TU057841 1.0 ml
EUR 2799.6

FCMTE2 3'UTR GFP Stable Cell Line

TU057842 1.0 ml
EUR 2799.6

FCMTE3 3'UTR GFP Stable Cell Line

TU057843 1.0 ml
EUR 2799.6

Fcmr Polyclonal Antibody

A56202
  • EUR 684.66
  • Ask for price
  • Ask for price
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Fcmr Antibody, HRP conjugated

1-CSB-PA007972LB01MO
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA

Fcmr Antibody, FITC conjugated

1-CSB-PA007972LC01MO
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA

Fcmr Antibody, Biotin conjugated

1-CSB-PA007972LD01MO
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA

FCMTE1 Protein Vector (Human) (pPM-C-HA)

PV078107 500 ng Ask for price

FCMTE2 Protein Vector (Human) (pPM-C-HA)

PV078111 500 ng Ask for price

FCMTE3 Protein Vector (Human) (pPM-C-HA)

PV078115 500 ng Ask for price

Fc Fragment Of IgM Receptor (FCMR) Antibody

20-abx109287
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fc Fragment of IgM Receptor (FCMR) Antibody

20-abx320226
  • EUR 360.00
  • EUR 292.80
  • 100 ul
  • 50 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody

20-abx339450
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody

20-abx339609
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

FCMTE1 Protein Vector (Human) (pPB-C-His)

PV078105 500 ng Ask for price

FCMTE1 Protein Vector (Human) (pPB-N-His)

PV078106 500 ng Ask for price

FCMTE1 Protein Vector (Human) (pPM-C-His)

PV078108 500 ng Ask for price

FCMTE2 Protein Vector (Human) (pPB-C-His)

PV078109 500 ng Ask for price

FCMTE2 Protein Vector (Human) (pPB-N-His)

PV078110 500 ng Ask for price

FCMTE2 Protein Vector (Human) (pPM-C-His)

PV078112 500 ng Ask for price

FCMTE3 Protein Vector (Human) (pPB-C-His)

PV078113 500 ng Ask for price

FCMTE3 Protein Vector (Human) (pPB-N-His)

PV078114 500 ng Ask for price

FCMTE3 Protein Vector (Human) (pPM-C-His)

PV078116 500 ng Ask for price

FCMTE1 Recombinant Protein (Human)

RP058578 100 ug Ask for price

FCMTE2 Recombinant Protein (Human)

RP058581 100 ug Ask for price

FCMTE3 Recombinant Protein (Human)

RP058584 100 ug Ask for price

FCMTE1 3'UTR Luciferase Stable Cell Line

TU007841 1.0 ml
EUR 2799.6

FCMTE2 3'UTR Luciferase Stable Cell Line

TU007842 1.0 ml
EUR 2799.6

FCMTE3 3'UTR Luciferase Stable Cell Line

TU007843 1.0 ml
EUR 2799.6

Fc Fragment Of IgM Receptor (FCMR) Antibody (HRP)

20-abx108239
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fc Fragment Of IgM Receptor (FCMR) Antibody (FITC)

20-abx106820
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fcmr Polyclonal Antibody, HRP Conjugated

A56203
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

Fc Fragment Of IgM Receptor (FCMR) Antibody (Biotin)

20-abx105401
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fcmr Polyclonal Antibody, FITC Conjugated

A56204
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

Polyclonal FCMR / FAIM3 Antibody (C-Terminus)

APR02365G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FCMR / FAIM3 (C-Terminus). This antibody is tested and proven to work in the following applications:

Fcmr Polyclonal Antibody, Biotin Conjugated

A56205
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV725231 1.0 ug DNA Ask for price

FCMTE1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV725235 1.0 ug DNA Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV725237 1.0 ug DNA Ask for price

FCMTE2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV725241 1.0 ug DNA Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV725243 1.0 ug DNA Ask for price

FCMTE3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV725247 1.0 ug DNA Ask for price

FCMTE1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV725236 1.0 ug DNA Ask for price

FCMTE2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV725242 1.0 ug DNA Ask for price

FCMTE3 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV725248 1.0 ug DNA Ask for price

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV725232 1.0 ug DNA Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV725238 1.0 ug DNA Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV725244 1.0 ug DNA Ask for price

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV725233 1.0 ug DNA Ask for price

FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV725234 1.0 ug DNA Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV725239 1.0 ug DNA Ask for price

FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV725240 1.0 ug DNA Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV725245 1.0 ug DNA Ask for price

FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV725246 1.0 ug DNA Ask for price

Fukuyama type congenital muscular dystrophy protein (FCMD) polyclonal antibody

ABP-PAB-10673 100 ug Ask for price

Our method detected parallel duplex formation as a function of length, chemical modifications, and pH, and at a sensitivity that required over 100 fold less concentration of sample than prior UV-melting methods. While parallel polyriboadenylic acid and poly-2′-O-methyl-adenylic acid homo-duplexes formed, we did not detect homo-duplexes of polydeoxyriboadenylic acid strands or poly-locked nucleic acid (LNA)-adenylic strands.

Importantly however, a poly-locked nucleic acid (LNA)-adenylic strand, as well as a poly-2′-O-methyl-adenylic strand formed a hetero-duplex with a polyriboadenylic strand. Overall, our work validates a new tool for studying parallel duplexes and reveals fundamental properties of poly(A) parallel duplex formation. Parallel duplexes may find use in DNA nanotechnology and in molecular biology applications such as a potential poly(rA) tail capture tool as an alternative to traditional oligo(dT) based purification.