Polyriboadenylic (poly(rA)) strands of sufficient length form parallel double helices in acidic and/or ammonium-containing conditions. Poly(rA) duplexes in acidic conditions are held together by A+-A+ base-pairing also involving base interactions with the phosphate backbone.
Traditional UV-melting studies of parallel poly(A) duplexes have typically examined homo-duplex formation of a single nucleic acid species in solution. We have adapted a technique utilizing a DNA nanoswitch that detects interaction of two different strands either with similar or differing lengths or modifications.
) SAM FCM (Alexa Fluor 488) |
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abx098904-60tests |
Abbexa |
60 tests |
EUR 1629.6 |
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 FCMR Antibody |
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1-CSB-PA007972ESR2HU |
Cusabio |
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Description: A polyclonal antibody against FCMR. Recognizes FCMR from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
 Fcmr Antibody |
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1-CSB-PA007972LA01MO |
Cusabio |
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Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB; Recommended dilution: WB:1:1000-1:5000 |
 (HRP)) Human IgM protein (Fc5mu) (HRP) |
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65C-CH0918 |
Fitzgerald |
1 mg |
EUR 868.8 |
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Description: Human IgM protein (Fc5mu) (HRP) conjugate |
 (FITC)) Human IgM protein (Fc5mu) (FITC) |
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65C-CH0917 |
Fitzgerald |
1 mg |
EUR 685.2 |
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Description: Human IgM protein (Fc5mu) (FITC) conjugate |
 (pORF)) FCMTE1 ORF Vector (Human) (pORF) |
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ORF019527 |
ABM |
1.0 ug DNA |
Ask for price |
 (pORF)) FCMTE2 ORF Vector (Human) (pORF) |
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ORF019528 |
ABM |
1.0 ug DNA |
Ask for price |
 (pORF)) FCMTE3 ORF Vector (Human) (pORF) |
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ORF019529 |
ABM |
1.0 ug DNA |
Ask for price |
 FCMTE1 3'UTR GFP Stable Cell Line |
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TU057841 |
ABM |
1.0 ml |
EUR 2799.6 |
 FCMTE2 3'UTR GFP Stable Cell Line |
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TU057842 |
ABM |
1.0 ml |
EUR 2799.6 |
 FCMTE3 3'UTR GFP Stable Cell Line |
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TU057843 |
ABM |
1.0 ml |
EUR 2799.6 |
 Fcmr Polyclonal Antibody |
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A56202 |
EpiGentek |
-
EUR 684.66
-
Ask for price
-
Ask for price
-
EUR 423.50
|
- 100 µg
- 20 ul
- 50 ul
- 100 ul
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 Fcmr Antibody, HRP conjugated |
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1-CSB-PA007972LB01MO |
Cusabio |
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Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is HRP conjugated. Tested in the following application: ELISA |
 Fcmr Antibody, FITC conjugated |
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1-CSB-PA007972LC01MO |
Cusabio |
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Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is FITC conjugated. Tested in the following application: ELISA |
 Fcmr Antibody, Biotin conjugated |
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1-CSB-PA007972LD01MO |
Cusabio |
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Description: A polyclonal antibody against Fcmr. Recognizes Fcmr from Mouse. This antibody is Biotin conjugated. Tested in the following application: ELISA |
 (pPM-C-HA)) FCMTE1 Protein Vector (Human) (pPM-C-HA) |
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PV078107 |
ABM |
500 ng |
Ask for price |
 (pPM-C-HA)) FCMTE2 Protein Vector (Human) (pPM-C-HA) |
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PV078111 |
ABM |
500 ng |
Ask for price |
 (pPM-C-HA)) FCMTE3 Protein Vector (Human) (pPM-C-HA) |
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PV078115 |
ABM |
500 ng |
Ask for price |
 Antibody) Fc Fragment Of IgM Receptor (FCMR) Antibody |
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20-abx109287 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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 Antibody) Fc Fragment of IgM Receptor (FCMR) Antibody |
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20-abx320226 |
Abbexa |
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Fc Fragment Of IgM Receptor (FCMR) Antibody |
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20-abx339450 |
Abbexa |
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Fc Fragment Of IgM Receptor (FCMR) Antibody |
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20-abx339609 |
Abbexa |
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 (pPB-C-His)) FCMTE1 Protein Vector (Human) (pPB-C-His) |
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PV078105 |
ABM |
500 ng |
Ask for price |
 (pPB-N-His)) FCMTE1 Protein Vector (Human) (pPB-N-His) |
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PV078106 |
ABM |
500 ng |
Ask for price |
 (pPM-C-His)) FCMTE1 Protein Vector (Human) (pPM-C-His) |
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PV078108 |
ABM |
500 ng |
Ask for price |
 (pPB-C-His)) FCMTE2 Protein Vector (Human) (pPB-C-His) |
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PV078109 |
ABM |
500 ng |
Ask for price |
 (pPB-N-His)) FCMTE2 Protein Vector (Human) (pPB-N-His) |
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PV078110 |
ABM |
500 ng |
Ask for price |
 (pPM-C-His)) FCMTE2 Protein Vector (Human) (pPM-C-His) |
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PV078112 |
ABM |
500 ng |
Ask for price |
 (pPB-C-His)) FCMTE3 Protein Vector (Human) (pPB-C-His) |
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PV078113 |
ABM |
500 ng |
Ask for price |
 (pPB-N-His)) FCMTE3 Protein Vector (Human) (pPB-N-His) |
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PV078114 |
ABM |
500 ng |
Ask for price |
 (pPM-C-His)) FCMTE3 Protein Vector (Human) (pPM-C-His) |
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PV078116 |
ABM |
500 ng |
Ask for price |
) FCMTE1 Recombinant Protein (Human) |
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RP058578 |
ABM |
100 ug |
Ask for price |
) FCMTE2 Recombinant Protein (Human) |
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RP058581 |
ABM |
100 ug |
Ask for price |
) FCMTE3 Recombinant Protein (Human) |
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RP058584 |
ABM |
100 ug |
Ask for price |
 FCMTE1 3'UTR Luciferase Stable Cell Line |
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TU007841 |
ABM |
1.0 ml |
EUR 2799.6 |
 FCMTE2 3'UTR Luciferase Stable Cell Line |
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TU007842 |
ABM |
1.0 ml |
EUR 2799.6 |
 FCMTE3 3'UTR Luciferase Stable Cell Line |
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TU007843 |
ABM |
1.0 ml |
EUR 2799.6 |
 Antibody (HRP)) Fc Fragment Of IgM Receptor (FCMR) Antibody (HRP) |
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20-abx108239 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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 Antibody (FITC)) Fc Fragment Of IgM Receptor (FCMR) Antibody (FITC) |
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20-abx106820 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
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 Fcmr Polyclonal Antibody, HRP Conjugated |
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A56203 |
EpiGentek |
-
EUR 684.66
-
EUR 302.50
-
EUR 423.50
|
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 Antibody (Biotin)) Fc Fragment Of IgM Receptor (FCMR) Antibody (Biotin) |
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20-abx105401 |
Abbexa |
-
EUR 493.20
-
EUR 2214.00
-
EUR 718.80
-
EUR 218.40
-
EUR 360.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
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 Fcmr Polyclonal Antibody, FITC Conjugated |
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A56204 |
EpiGentek |
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EUR 684.66
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EUR 302.50
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EUR 423.50
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) Polyclonal FCMR / FAIM3 Antibody (C-Terminus) |
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APR02365G |
Leading Biology |
0.05mg |
EUR 580.8 |
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Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human FCMR / FAIM3 (C-Terminus). This antibody is tested and proven to work in the following applications: |
 Fcmr Polyclonal Antibody, Biotin Conjugated |
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A56205 |
EpiGentek |
-
EUR 684.66
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EUR 302.50
-
EUR 423.50
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 (CMV) (pLenti-GIII-CMV)) FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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LV725231 |
ABM |
1.0 ug DNA |
Ask for price |
 (UbC) (pLenti-GIII-UbC)) FCMTE1 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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LV725235 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV)) FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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LV725237 |
ABM |
1.0 ug DNA |
Ask for price |
 (UbC) (pLenti-GIII-UbC)) FCMTE2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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LV725241 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV)) FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV) |
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LV725243 |
ABM |
1.0 ug DNA |
Ask for price |
 (UbC) (pLenti-GIII-UbC)) FCMTE3 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC) |
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LV725247 |
ABM |
1.0 ug DNA |
Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) FCMTE1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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LV725236 |
ABM |
1.0 ug DNA |
Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) FCMTE2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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LV725242 |
ABM |
1.0 ug DNA |
Ask for price |
 (EF1a) (pLenti-GIII-EF1a)) FCMTE3 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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LV725248 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-C-term-HA)) FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV725232 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-C-term-HA)) FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV725238 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-C-term-HA)) FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV725244 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV725233 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) FCMTE1 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV725234 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV725239 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) FCMTE2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV725240 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)) FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV725245 |
ABM |
1.0 ug DNA |
Ask for price |
 (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)) FCMTE3 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV725246 |
ABM |
1.0 ug DNA |
Ask for price |
 polyclonal antibody) Fukuyama type congenital muscular dystrophy protein (FCMD) polyclonal antibody |
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ABP-PAB-10673 |
Allele Biotech |
100 ug |
Ask for price |
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Our method detected parallel duplex formation as a function of length, chemical modifications, and pH, and at a sensitivity that required over 100 fold less concentration of sample than prior UV-melting methods. While parallel polyriboadenylic acid and poly-2′-O-methyl-adenylic acid homo-duplexes formed, we did not detect homo-duplexes of polydeoxyriboadenylic acid strands or poly-locked nucleic acid (LNA)-adenylic strands.
Importantly however, a poly-locked nucleic acid (LNA)-adenylic strand, as well as a poly-2′-O-methyl-adenylic strand formed a hetero-duplex with a polyriboadenylic strand. Overall, our work validates a new tool for studying parallel duplexes and reveals fundamental properties of poly(A) parallel duplex formation. Parallel duplexes may find use in DNA nanotechnology and in molecular biology applications such as a potential poly(rA) tail capture tool as an alternative to traditional oligo(dT) based purification.
