Parallel poly(A) homo- and hetero-duplex formation detection with an adapted DNA nanoswitch technique.

Polyriboadenylic (poly(rA)) strands of sufficient length form parallel double helices in acidic and/or ammonium-containing conditions. Poly(rA) duplexes in acidic conditions are held together by A+-A+ base-pairing also involving base interactions with the phosphate backbone.

Traditional UV-melting studies of parallel poly(A) duplexes have typically examined homo-duplex formation of a single nucleic acid species in solution. We have adapted a technique utilizing a DNA nanoswitch that detects interaction of two different strands either with similar or differing lengths or modifications.

SAM FCM (Alexa Fluor 647)

abx098902-100tests 100 tests
EUR 1233
  • Shipped within 5-10 working days.

SAM FCM (Alexa Fluor 488)

abx098904-60tests 60 tests
EUR 1358
  • Shipped within 5-10 working days.

Fluorescent Carboxyl Superparamagnetic Particles

FCM-02552--2H 2mL
EUR 283
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-02556-2 2 mL
EUR 223
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-02558--2H 2mL
EUR 283
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-0552-2H 2mL
EUR 249
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-0556-2 2 mL
EUR 223
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-0558-2H 2mL
EUR 243
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-0852-2 2 mL
EUR 223
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-0856-2 2 mL
EUR 223
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-100052--2 2 mL
EUR 330
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-10052-2 2 mL
EUR 279
Description: Fluorescent Carboxyl Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Fluorescent Carboxyl Magnetic Particles

FCM-1045-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-1052-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-1056-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-1058-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-1065-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-1068-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-1070-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-200052--2 2 mL
EUR 330
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-20052-2 2 mL
EUR 304
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-2042-2 2 mL
EUR 218
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-2052-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-2058-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-2062-2 2 mL
EUR 212
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-2070-2 2 mL
EUR 259
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-4052-2 2 mL
EUR 259
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-4056-2 2 mL
EUR 259
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-4058-2 2 mL
EUR 259
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-50052-2 2 mL
EUR 330
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-5052-2 2 mL
EUR 263
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-7041-2 2 mL
EUR 223
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-7067-2 2 mL
EUR 223
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-8052-2 2 mL
EUR 273
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-8056-2 2 mL
EUR 273
Description: Fluorescent Carboxyl Magnetic Particles

Fluorescent Carboxyl Magnetic Particles

FCM-8058-2 2 mL
EUR 273
Description: Fluorescent Carboxyl Magnetic Particles

Our method detected parallel duplex formation as a function of length, chemical modifications, and pH, and at a sensitivity that required over 100 fold less concentration of sample than prior UV-melting methods. While parallel polyriboadenylic acid and poly-2′-O-methyl-adenylic acid homo-duplexes formed, we did not detect homo-duplexes of polydeoxyriboadenylic acid strands or poly-locked nucleic acid (LNA)-adenylic strands.

Importantly however, a poly-locked nucleic acid (LNA)-adenylic strand, as well as a poly-2′-O-methyl-adenylic strand formed a hetero-duplex with a polyriboadenylic strand. Overall, our work validates a new tool for studying parallel duplexes and reveals fundamental properties of poly(A) parallel duplex formation. Parallel duplexes may find use in DNA nanotechnology and in molecular biology applications such as a potential poly(rA) tail capture tool as an alternative to traditional oligo(dT) based purification.