Molecular analysis of H&E- and Papanicolau-stained samples-systematic review.

Molecular pathology allows the identification of causative agents in infectious diseases and detection of biomarkers important for prediction of disease susceptibility, diagnosis and personalized therapy. Accordingly, nucleic acid-based methods have gained a special role in clinical laboratories particularly to evaluate solid and hematological tumors. Extraction of nucleic acids is commonly performed in microdissected formalin-fixed paraffin-embedded (FFPE) or cytological samples that had been previously evaluated through the use of hematoxylin and eosin (H&E) or Papanicolau (Pap) stains, respectively.

Although the effect of both stains on nucleic acids integrity has been explored by several authors, the results are not consistent and require further examination. Accordingly, the goal of this review was to assess the influence of H&E and Pap stains on DNA and RNA integrity and to address the mechanism by which each staining compromises molecular based-analysis. The analyzed studies demonstrate that H&E- and Pap-staining result in low DNA recovery and some degree of DNA fragmentation.

FCGR3B

MBS8561528-01mLAF405L 0.1mL(AF405L)
EUR 565

FCGR3B

MBS8561528-01mLAF405S 0.1mL(AF405S)
EUR 565

FCGR3B

MBS8561528-01mLAF610 0.1mL(AF610)
EUR 565

FCGR3B

MBS8561528-01mLAF635 0.1mL(AF635)
EUR 565

FCGR3B

MBS8561528-02mL 0.2mL
EUR 345

FCGR3B

MBS8536555-01mL 0.1mL
EUR 325

FCGR3B

MBS8536555-01mLAF405L 0.1mL(AF405L)
EUR 565

FCGR3B

MBS8536555-01mLAF405S 0.1mL(AF405S)
EUR 565

FCGR3B

MBS8536555-01mLAF610 0.1mL(AF610)
EUR 565

FCGR3B

MBS8536555-01mLAF635 0.1mL(AF635)
EUR 565

FCGR3B siRNA

20-abx916694
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

FCGR3B Antibody

E97894 100ul
EUR 255
Description: Available in various conjugation types.

FCGR3B Antibody

E309161 200ul
EUR 275
Description: Available in various conjugation types.

FCGR3B Antibody

1-CSB-PA008544LA01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against FCGR3B. Recognizes FCGR3B from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

FCGR3B Antibody

K52021M01B09C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

FCGR3B Antibody

K52021M01B09C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

FCGR3B Antibody

K52021M01B09C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

FCGR3B Antibody

K52021M12F02C-100ug 100 ug
EUR 320
Description: Monoclonal Antibody

FCGR3B Antibody

K52021M12F02C-1mg 1 mg
EUR 1600
Description: Monoclonal Antibody

FCGR3B Antibody

K52021M12F02C-50ug 50 ug
EUR 224
Description: Monoclonal Antibody

FCGR3B Antibody

MBS7108864-005mg 0.05mg
EUR 190

FCGR3B Antibody

MBS7108864-01mg 0.1mg
EUR 270

FCGR3B Antibody

MBS7108864-5x01mg 5x0.1mg
EUR 1205

FCGR3A / FCGR3B Antibody

20-abx323190
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

FCGR3A/FCGR3B Antibody

1-CSB-PA676166
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against FCGR3A/FCGR3B. Recognizes FCGR3A/FCGR3B from Human. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC-p:1:50-300, ELISA:1:10000-20000

FCGR3A / FCGR3B Antibody

abx323190-100g 100 µg
EUR 250

FCGR3A / FCGR3B Antibody

abx323190-50g 50 µg
EUR 187.5

FCGR3A/FCGR3B Antibody

MBS7122883-005mg 0.05mg
EUR 150

FCGR3A/FCGR3B Antibody

MBS7122883-01mg 0.1mg
EUR 190

FCGR3A/FCGR3B Antibody

MBS7122883-5x01mg 5x0.1mg
EUR 845

FCGR3B Rabbit pAb

A7894-100ul 100 ul
EUR 369.6

FCGR3B Rabbit pAb

A7894-200ul 200 ul
EUR 550.8

FCGR3B Rabbit pAb

A7894-20ul 20 ul
EUR 219.6

FCGR3B Rabbit pAb

A7894-50ul 50 ul
EUR 267.6

FCGR3B Rabbit pAb

E2507894 100ul
EUR 225
Description: Available in various conjugation types.

FCGR3B Rabbit pAb

E2513980 100ul
EUR 225
Description: Available in various conjugation types.

FCGR3B Rabbit pAb

A13980-100ul 100 ul
EUR 369.6

FCGR3B Rabbit pAb

A13980-200ul 200 ul
EUR 550.8

FCGR3B Rabbit pAb

A13980-20ul 20 ul
EUR 219.6

FCGR3B Rabbit pAb

A13980-50ul 50 ul
EUR 267.6

FCGR3B cDNA Clone

MBS1277326-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

FCGR3B cDNA Clone

MBS1277326-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

FCGR3B Rabbit pAb

MBS8549283-01mL 0.1mL
EUR 305

FCGR3B Rabbit pAb

MBS8549283-01mLAF405L 0.1mL(AF405L)
EUR 565

FCGR3B Rabbit pAb

MBS8549283-01mLAF405S 0.1mL(AF405S)
EUR 565

FCGR3B Rabbit pAb

MBS8549283-01mLAF610 0.1mL(AF610)
EUR 565

FCGR3B Rabbit pAb

MBS8549283-01mLAF635 0.1mL(AF635)
EUR 565

FCGR3B Rabbit pAb

MBS8547112-01mL 0.1mL
EUR 305

FCGR3B Rabbit pAb

MBS8547112-01mLAF405L 0.1mL(AF405L)
EUR 565

FCGR3B Rabbit pAb

MBS8547112-01mLAF405S 0.1mL(AF405S)
EUR 565

FCGR3B Rabbit pAb

MBS8547112-01mLAF610 0.1mL(AF610)
EUR 565

FCGR3B Rabbit pAb

MBS8547112-01mLAF635 0.1mL(AF635)
EUR 565

FCGR3B Rabbit pAb

A7894 500μL
EUR 1246.96

FCGR3B Rabbit pAb

A13980 100μL
EUR 262.15

FCGR3B siRNA (Human)

MBS8227202-15nmol 15nmol
EUR 405

FCGR3B siRNA (Human)

MBS8227202-30nmol 30nmol
EUR 565

FCGR3B siRNA (Human)

MBS8227202-5x30nmol 5x30nmol
EUR 2450

FCGR3B cloning plasmid

CSB-CL008544HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the FCGR3B gene.

FCGR3B Polyclonal Antibody

28411 100ul
EUR 439

FCGR3B Polyclonal Antibody

28411-100ul 100ul
EUR 302.4

FCGR3B Polyclonal Antibody

28411-50ul 50ul
EUR 224.4

FCGR3B Polyclonal Antibody

31372-100ul 100ul
EUR 302.4

FCGR3B Polyclonal Antibody

31372-50ul 50ul
EUR 224.4

FCGR3B Polyclonal Antibody

E913980 100ul
EUR 225
Description: Available in various conjugation types.

FCGR3B Polyclonal Antibody

E-AB-64883-120uL 120uL
EUR 320
Description: Unconjugated

FCGR3B Polyclonal Antibody

E-AB-64883-200uL 200uL
EUR 530
Description: Unconjugated

FCGR3B Polyclonal Antibody

E-AB-64883-60uL 60uL
EUR 200
Description: Unconjugated

FCGR3B Polyclonal Antibody

E-AB-64883-each each Ask for price
Description: Unconjugated

FCGR3B Polyclonal Antibody

MBS9133990-002mL 0.02mL
EUR 200

FCGR3B Polyclonal Antibody

MBS9133990-005mL 0.05mL
EUR 255

FCGR3B Polyclonal Antibody

MBS9133990-01mL 0.1mL
EUR 345

FCGR3B Polyclonal Antibody

MBS9133990-02mL 0.2mL
EUR 545

FCGR3B Polyclonal Antibody

MBS9133990-5x02mL 5x0.2mL
EUR 2265

FCGR3B Polyclonal Antibody

MBS9128928-002mL 0.02mL
EUR 200

FCGR3B Polyclonal Antibody

MBS9128928-005mL 0.05mL
EUR 255

FCGR3B Polyclonal Antibody

MBS9128928-01mL 0.1mL
EUR 345

FCGR3B Polyclonal Antibody

MBS9128928-02mL 0.2mL
EUR 545

FCGR3B Polyclonal Antibody

MBS9128928-5x02mL 5x0.2mL
EUR 2265

FCGR3B Polyclonal Antibody

MBS8568685-01mL 0.1mL
EUR 305

FCGR3B Polyclonal Antibody

MBS8568685-01mLAF405L 0.1mL(AF405L)
EUR 565

FCGR3B Polyclonal Antibody

MBS8568685-01mLAF405S 0.1mL(AF405S)
EUR 565

FCGR3B Polyclonal Antibody

MBS8568685-01mLAF610 0.1mL(AF610)
EUR 565

FCGR3B Polyclonal Antibody

MBS8568685-01mLAF635 0.1mL(AF635)
EUR 565

FCGR3B Polyclonal Antibody

MBS2541242-006mL 0.06mL
EUR 180

FCGR3B Polyclonal Antibody

MBS2541242-012mL 0.12mL
EUR 260

FCGR3B Polyclonal Antibody

MBS2541242-02mL 0.2mL
EUR 405

FCGR3B Polyclonal Antibody

MBS2541242-5x02mL 5x0.2mL
EUR 1725

FCGR3B Polyclonal Antibody

MBS2555258-006mL 0.06mL
EUR 190

FCGR3B Polyclonal Antibody

MBS2555258-012mL 0.12mL
EUR 265

FCGR3B Polyclonal Antibody

MBS2555258-02mL 0.2mL
EUR 415

FCGR3B Polyclonal Antibody

MBS2555258-5x02mL 5x0.2mL
EUR 1835

FCGR3B Polyclonal Antibody

MBS9434203-005mL 0.05mL
EUR 300

FCGR3B Polyclonal Antibody

MBS9434203-01mL 0.1mL
EUR 390

FCGR3B Polyclonal Antibody

MBS9434203-5x01mL 5x0.1mL
EUR 1610

FCGR3B Polyclonal Antibody

MBS9432005-005mL 0.05mL
EUR 300

FCGR3B Polyclonal Antibody

MBS9432005-01mL 0.1mL
EUR 390

FCGR3B Polyclonal Antibody

MBS9432005-5x01mL 5x0.1mL
EUR 1610

FCGR3B Polyclonal Antibody

RD81666A-120uL 120μL
EUR 360
Description: The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1.

FCGR3B Polyclonal Antibody

RD81666A-200uL 200μL
EUR 630
Description: The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1.

FCGR3B Polyclonal Antibody

RD81666A-60uL 60μL
EUR 204
Description: The protein encoded by this gene is a low affinity receptor for the Fc region of gamma immunoglobulins (IgG). The encoded protein acts as a monomer and can bind either monomeric or aggregated IgG. This gene may function to capture immune complexes in the peripheral circulation. Several transcript variants encoding different isoforms have been found for this gene. A highly-similar gene encoding a related protein is also found on chromosome 1.

Rat FCGR3b ELISA Kit

ERF0073 96Tests
EUR 625.2

Goat FCGR3b ELISA Kit

EGTF0073 96Tests
EUR 625.2

FCGR3B, Avi-His-Tag

79016 100 µg
EUR 320
Description: Human FCGR3B, known as CD16b, and FCG3, GenBank Accession No. NM_000570.4, a.a. 20-208, with C-terminal Avi™-His-Tag, expressed in a HEK293 cell expression system. This protein runs at a higher MW by SDS-PAGE due to glycosylation. MW = 25 kDa.

Human FCGR3b ELISA Kit

EHF0073 96Tests
EUR 625.2

Human FCGR3B ELISA Kit

ELA-E9986h 96tests
EUR 696

Human FCGR3B ELISA KIT

ELI-07765h 96tests
EUR 696

Sheep FCGR3b ELISA Kit

ESF0073 96Tests
EUR 625.2

Mouse FCGR3b ELISA Kit

EMF0073 96Tests
EUR 625.2

Bovine FCGR3b ELISA Kit

EBF0073 96Tests
EUR 625.2

Canine FCGR3b ELISA Kit

ECF0073 96Tests
EUR 625.2

Rabbit FCGR3b ELISA Kit

ERTF0073 96Tests
EUR 625.2

Monkey FCGR3b ELISA Kit

EMKF0073 96Tests
EUR 625.2

Chicken FCGR3b ELISA Kit

ECKF0073 96Tests
EUR 625.2

Porcine FCGR3b ELISA Kit

EPF0073 96Tests
EUR 625.2

FCGR3B Antibody (N-term)

MBS9204715-008mL 0.08mL
EUR 210

FCGR3B Antibody (N-term)

MBS9204715-04mL 0.4mL
EUR 430

FCGR3B Antibody (N-term)

MBS9204715-5x04mL 5x0.4mL
EUR 1910

Anserini FCGR3b ELISA Kit

EAF0073 96Tests
EUR 625.2

Human FCGR3B shRNA Plasmid

20-abx951549
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

FCGR3B Antibody, HRP conjugated

1-CSB-PA008544LB01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against FCGR3B. Recognizes FCGR3B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

FCGR3B Antibody, HRP conjugated

MBS7108865-005mg 0.05mg
EUR 190

FCGR3B Antibody, HRP conjugated

MBS7108865-01mg 0.1mg
EUR 270

FCGR3B Antibody, HRP conjugated

MBS7108865-5x01mg 5x0.1mg
EUR 1205

Human CD16b Recombinant (FcgR3b)

MBS553103-002mg 0.02mg
EUR 245

Human CD16b Recombinant (FcgR3b)

MBS553103-01mg 0.1mg
EUR 585

Human CD16b Recombinant (FcgR3b)

MBS553103-05mg 0.5mg
EUR 2025

FCGR3B (untagged)-Human Fc fragment of IgG, low affinity IIIb, receptor (CD16b) (FCGR3B)

SC319729 10 µg Ask for price

FCGR3B Antibody, FITC conjugated

1-CSB-PA008544LC01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against FCGR3B. Recognizes FCGR3B from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

OACD03458-1ML - FCGR3B Antibody

OACD03458-1ML 1ml
EUR 639

FCGR3B Antibody, FITC conjugated

MBS7108866-005mg 0.05mg
EUR 190

FCGR3B Antibody, FITC conjugated

MBS7108866-01mg 0.1mg
EUR 270

FCGR3B Antibody, FITC conjugated

MBS7108866-5x01mg 5x0.1mg
EUR 1205

OAAN02495-50UL - FCGR3B Antibody

OAAN02495-50UL 50ul
EUR 149

FCGR3B Recombinant Protein (Human)

RP039058 100 ug Ask for price

Recombinant Human FCGR3B Protein

MBS8249025-001mg 0.01mg
EUR 230

Recombinant Human FCGR3B Protein

MBS8249025-005mg 0.05mg
EUR 480

Recombinant Human FCGR3B Protein

MBS8249025-05mg 0.5mg
EUR 2825

Recombinant Human FCGR3B Protein

MBS8249025-5x05mg 5x0.5mg
EUR 12640

FCGR3B Antibody, Biotin conjugated

1-CSB-PA008544LD01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against FCGR3B. Recognizes FCGR3B from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OACD03458-100UL - FCGR3B Antibody

OACD03458-100UL 100ul
EUR 189

OAAN02495-100UL - FCGR3B Antibody

OAAN02495-100UL 100ul
EUR 249

OAAN02495-200UL - FCGR3B Antibody

OAAN02495-200UL 200ul
EUR 389

FCGR3B Antibody, Biotin conjugated

MBS7108867-005mg 0.05mg
EUR 190

FCGR3B Antibody, Biotin conjugated

MBS7108867-01mg 0.1mg
EUR 270

FCGR3B Antibody, Biotin conjugated

MBS7108867-5x01mg 5x0.1mg
EUR 1205

FcGR3B (CD16B) CHO Cell Line

78333 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full length human CD16B (also known as FCGR3B, FcγRIIIb, ref. seq. NM_000570) under the control of the CMV promoter.  The surface expression of CD16B was validated by flow cytometry.

FCGR3B (N-term) Rabbit pAb

E2617533 100ul
EUR 225
Description: Available in various conjugation types.

FCGR3B (N-Term) Rabbit pAb

MBS8555888-01mL 0.1mL
EUR 305

FCGR3B (N-Term) Rabbit pAb

MBS8555888-01mLAF405L 0.1mL(AF405L)
EUR 565

FCGR3B (N-Term) Rabbit pAb

MBS8555888-01mLAF405S 0.1mL(AF405S)
EUR 565

FCGR3B (N-Term) Rabbit pAb

MBS8555888-01mLAF610 0.1mL(AF610)
EUR 565

FCGR3B (N-Term) Rabbit pAb

MBS8555888-01mLAF635 0.1mL(AF635)
EUR 565

Guinea Pig FCGR3b ELISA Kit

EGF0073 96Tests
EUR 625.2

FCGR3B (GFP-tagged) - Human Fc fragment of IgG, low affinity IIIb, receptor (CD16b) (FCGR3B)

RG204749 10 µg Ask for price

Human FCGR3B Protein, Avi Tag

E40KMPH6594 20ug
EUR 495

Human FCGR3B Protein, His Tag

E40KMPH6606 20ug
EUR 495

Mouse FCGR3B Protein, His Tag

E40MOP1278 20ug
EUR 495

Human FCGR3B Protein, His Tag

E40KMPH2519 20ug
EUR 495

Human FCGR3B Protein, His Tag

E40KMPH2520 20ug
EUR 495

Human FCGR3B Protein, His Tag

E40KMPH2521 20ug
EUR 495

Human FCGR3B Protein, His Tag

E40KMPH2522 20ug
EUR 495

FCGR3B ORF Vector (Human) (pORF)

ORF013020 1.0 ug DNA
EUR 424.8

FCGR3B Polyclonal Conjugated Antibody

C28411 100ul
EUR 476.4

FCGR3B Polyclonal Conjugated Antibody

C31372 100ul
EUR 476.4

FCGR3B Polyclonal Conjugated Antibody

MBS9442545-01mLAF350 0.1mL(AF350)
EUR 480

FCGR3B Polyclonal Conjugated Antibody

MBS9442545-01mLAF405 0.1mL(AF405)
EUR 480

Additionally, it is concluded that hemalum inhibits PCR by interfering with DNA extraction, preventing DNA polymerase attachment and possibly by rescuing divalent cations. Accordingly, proper sample purification and adjustment of PCR conditions are of key importance to achieve satisfactory results by PCR in H&E- and Pap-stained samples.

Furthermore, although H&E results in RNA fragmentation, it is possible to perform expression analysis in H&E-stained frozen sections, using RNase-free conditions, low amounts of hematoxylin and a rapid protocol from sample collection to RNA analysis. It The effect of Pap-staining on RNA integrity remains to be determined.