Nucleic acid amplification based mostly detection performs an vital function in meals security, environmental monitoring and scientific analysis. However, conventional nucleic acid detection course of includes transferring liquid from one tube to a different by pipetting It requires skilled individuals, geared up labs and consumes numerous time.
The superb nucleic acid detection is integrated, closed, simplified and automated. Magnetic particles actuated by magnetic fields can effectively adsorb nucleic acids and promote integrated nucleic acid assays without pipetting pushed by pumps and centrifuges. We will comprehensively evaluate magnetic particles assisted integrated system for nucleic acid detection and hope it could possibly encourage additional associated research.

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting
An ultrasensitive and particular point-of-care CRISPR/Cas12 based mostly lateral move biosensor for the fast detection of nucleic acids.
CRISPR/Cas techniques have displayed exceptional potential in creating novel biosensing functions for nucleic acid detection owing to the collateral cleavage exercise of Cas effector proteins (Cas12, Cas13, and many others.). Despite super progress lately, the prevailing CRISPR/Cas based mostly biosensing platforms have a number of limitations, together with reliance on correct amplification strategies, costly fluorescence detection tools, or lateral move biosensor (LFB).
EDEM1 Antibody |
47091-100ul |
SAB |
100ul |
EUR 302.4 |
EDEM1 siRNA |
20-abx914949 |
Abbexa |
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EDEM1 siRNA |
20-abx914950 |
Abbexa |
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EDEM1 antibody |
70R-6871 |
Fitzgerald |
50 ug |
EUR 560.4 |
Description: Rabbit polyclonal EDEM1 antibody raised against the N terminal of EDEM1 |
EDEM1 Antibody |
1-CSB-PA838793LA01HU |
Cusabio |
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Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:200-1:500 |
EDEM1 Antibody |
3904-100 |
Biovision |
each |
EUR 444 |
EDEM1 Antibody |
3904-30T |
Biovision |
each |
EUR 175.2 |
EDEM1 Blocking Peptide |
33R-5674 |
Fitzgerald |
100 ug |
EUR 216 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EDEM1 antibody, catalog no. 70R-6871 |
EDEM1 Blocking Peptide |
20-abx161085 |
Abbexa |
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EDEM1 Polyclonal Antibody |
ABP58453-003ml |
Abbkine |
0.03ml |
EUR 189.6 |
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Description: A polyclonal antibody for detection of EDEM1 from Human, Mouse. This EDEM1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EDEM1 protein |
EDEM1 Polyclonal Antibody |
ABP58453-01ml |
Abbkine |
0.1ml |
EUR 346.8 |
|
Description: A polyclonal antibody for detection of EDEM1 from Human, Mouse. This EDEM1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EDEM1 protein |
EDEM1 Polyclonal Antibody |
ABP58453-02ml |
Abbkine |
0.2ml |
EUR 496.8 |
|
Description: A polyclonal antibody for detection of EDEM1 from Human, Mouse. This EDEM1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EDEM1 protein |
EDEM1 Conjugated Antibody |
C47091 |
SAB |
100ul |
EUR 476.4 |
EDEM1 Conjugated Antibody |
C45971 |
SAB |
100ul |
EUR 476.4 |
EDEM1 Blocking Peptide |
DF9502-BP |
Affbiotech |
1mg |
EUR 234 |
EDEM1 Polyclonal Antibody |
ES9648-100ul |
ELK Biotech |
100ul |
EUR 334.8 |
Description: A Rabbit Polyclonal antibody against EDEM1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
EDEM1 Polyclonal Antibody |
ES9648-50ul |
ELK Biotech |
50ul |
EUR 248.4 |
Description: A Rabbit Polyclonal antibody against EDEM1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA |
Anti-EDEM1 antibody |
STJ190806 |
St John's Laboratory |
200 µl |
EUR 236.4 |
Description: Unconjugated Rabbit polyclonal to EDEM1 |
Anti-EDEM1 (2D3) |
YF-MA16973 |
Abfrontier |
100 ug |
EUR 435.6 |
Description: Mouse monoclonal to EDEM1 |
EDEM1 Blocking Peptide |
3904BP-50 |
Biovision |
each |
EUR 183.6 |
EDEM1 Antibody, HRP conjugated |
1-CSB-PA838793LB01HU |
Cusabio |
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|
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Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
EDEM1 Antibody, FITC conjugated |
1-CSB-PA838793LC01HU |
Cusabio |
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Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
EDEM1 Antibody, Biotin conjugated |
1-CSB-PA838793LD01HU |
Cusabio |
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Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Mouse EDEM1 shRNA Plasmid |
20-abx980418 |
Abbexa |
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Human EDEM1 shRNA Plasmid |
20-abx956466 |
Abbexa |
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EDEM1 Recombinant Protein (Human) |
RP056046 |
ABM |
100 ug |
Ask for price |
EDEM1 Recombinant Protein (Mouse) |
RP130853 |
ABM |
100 ug |
Ask for price |
EDEM1 ORF Vector (Human) (pORF) |
ORF018683 |
ABM |
1.0 ug DNA |
EUR 486 |
Edem1 ORF Vector (Mouse) (pORF) |
ORF043619 |
ABM |
1.0 ug DNA |
EUR 607.2 |
Polyclonal EDEM1 antibody - N-terminal region |
AMM07002G |
Leading Biology |
0.05mg |
EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EDEM1 - N-terminal region. This antibody is tested and proven to work in the following applications: |
EDEM1 sgRNA CRISPR Lentivector set (Human) |
K0653401 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
Edem1 sgRNA CRISPR Lentivector set (Mouse) |
K4015601 |
ABM |
3 x 1.0 ug |
EUR 406.8 |
EDEM1 sgRNA CRISPR Lentivector (Human) (Target 1) |
K0653402 |
ABM |
1.0 ug DNA |
EUR 184.8 |
EDEM1 sgRNA CRISPR Lentivector (Human) (Target 2) |
K0653403 |
ABM |
1.0 ug DNA |
EUR 184.8 |
EDEM1 sgRNA CRISPR Lentivector (Human) (Target 3) |
K0653404 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Edem1 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
K4015602 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Edem1 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
K4015603 |
ABM |
1.0 ug DNA |
EUR 184.8 |
Edem1 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
K4015604 |
ABM |
1.0 ug DNA |
EUR 184.8 |
EDEM1 Protein Vector (Human) (pPB-C-His) |
PV074729 |
ABM |
500 ng |
EUR 662.4 |
EDEM1 Protein Vector (Human) (pPB-N-His) |
PV074730 |
ABM |
500 ng |
EUR 662.4 |
EDEM1 Protein Vector (Human) (pPM-C-HA) |
PV074731 |
ABM |
500 ng |
EUR 662.4 |
EDEM1 Protein Vector (Human) (pPM-C-His) |
PV074732 |
ABM |
500 ng |
EUR 662.4 |
EDEM1 Protein Vector (Mouse) (pPB-C-His) |
PV174474 |
ABM |
500 ng |
EUR 723.6 |
EDEM1 Protein Vector (Mouse) (pPB-N-His) |
PV174475 |
ABM |
500 ng |
EUR 723.6 |
EDEM1 Protein Vector (Mouse) (pPM-C-HA) |
PV174476 |
ABM |
500 ng |
EUR 723.6 |
EDEM1 Protein Vector (Mouse) (pPM-C-His) |
PV174477 |
ABM |
500 ng |
EUR 723.6 |
EDEM1 3'UTR Luciferase Stable Cell Line |
TU006559 |
ABM |
1.0 ml |
EUR 2799.6 |
Edem1 3'UTR GFP Stable Cell Line |
TU253776 |
ABM |
1.0 ml |
Ask for price |
Edem1 3'UTR Luciferase Stable Cell Line |
TU105597 |
ABM |
1.0 ml |
Ask for price |
EDEM1 3'UTR GFP Stable Cell Line |
TU056559 |
ABM |
1.0 ml |
EUR 2799.6 |
Edem1 3'UTR Luciferase Stable Cell Line |
TU203776 |
ABM |
1.0 ml |
Ask for price |
Edem1 3'UTR GFP Stable Cell Line |
TU155597 |
ABM |
1.0 ml |
Ask for price |
ER Degradation-Enhancing Alpha-Mannosidase-Like Protein 1 (EDEM1) Antibody |
20-abx121085 |
Abbexa |
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EUR 360.00
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EUR 526.80
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EUR 226.80
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ER Degradation-Enhancing Alpha-Mannosidase-Like Protein 1 (EDEM1) Antibody |
20-abx215066 |
Abbexa |
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EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
K0653405 |
ABM |
3 x 1.0 ug |
EUR 451.2 |
Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
K4015605 |
ABM |
3 x 1.0 ug |
EUR 451.2 |
EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
K0653406 |
ABM |
1.0 ug DNA |
EUR 200.4 |
EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
K0653407 |
ABM |
1.0 ug DNA |
EUR 200.4 |
EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
K0653408 |
ABM |
1.0 ug DNA |
EUR 200.4 |
Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
K4015606 |
ABM |
1.0 ug DNA |
EUR 200.4 |
Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
K4015607 |
ABM |
1.0 ug DNA |
EUR 200.4 |
Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
K4015608 |
ABM |
1.0 ug DNA |
EUR 200.4 |
Herein, we report a easy, cheap, and ultrasensitive DNA probe based mostly LFB with CRISPR/Cas and loop-mediated Isothermal Amplification (particularly CIA). The idea behind this strategy is a non-detectable take a look at line on the LFB when the Cas effector protein collaterally cleaves the cognate goal and an ssDNA reporter sequence. The CIA based mostly LFB can detect as little as a single copy cloned Pseudomonas aeruginosa acyltransferase gene, 1 cfu/ml plasmid containing E. coli DH5α pure cultures, in addition to scientific samples without DNA extraction/purification or superior apparatuses. No cross-reactivity with different non-target micro organism was noticed. The bare eye end result readout was obtained in 15 min of LAMP amplification, 30 min of Cas12 response, and 5 min of LFB readout. This platform is powerful and of low price for on-site testing.