Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Nucleic acid amplification based mostly detection performs an vital function in meals security, environmental monitoring and scientific analysis. However, conventional nucleic acid detection course of includes transferring liquid from one tube to a different by pipetting It requires skilled individuals, geared up labs and consumes numerous time.

The superb nucleic acid detection is integrated, closed, simplified and automated. Magnetic particles actuated by magnetic fields can effectively adsorb nucleic acids and promote integrated nucleic acid assays without pipetting pushed by pumps and centrifuges. We will comprehensively evaluate magnetic particles assisted integrated system for nucleic acid detection and hope it could possibly encourage additional associated research.

An ultrasensitive and particular point-of-care CRISPR/Cas12 based mostly lateral move biosensor for the fast detection of nucleic acids.

CRISPR/Cas techniques have displayed exceptional potential in creating novel biosensing functions for nucleic acid detection owing to the collateral cleavage exercise of Cas effector proteins (Cas12, Cas13, and many others.). Despite super progress lately, the prevailing CRISPR/Cas based mostly biosensing platforms have a number of limitations, together with reliance on correct amplification strategies, costly fluorescence detection tools, or lateral move biosensor (LFB).

EDEM1 Antibody
47091-100ul	SAB	100ul	EUR 302.4

EDEM1 Antibody
ABD9502	Lifescience Market	100 ug	EUR 525.6

EDEM1 siRNA
20-abx914949	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

EDEM1 siRNA
20-abx914950	Abbexa	EUR 661.20 EUR 878.40	15 nmol 30 nmol

EDEM1 antibody
70R-6871	Fitzgerald	50 ug	EUR 560.4
Description: Rabbit polyclonal EDEM1 antibody raised against the N terminal of EDEM1

EDEM1 Antibody
1-CSB-PA838793LA01HU	Cusabio	EUR 380.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:200-1:500

EDEM1 Antibody
DF9502	Affbiotech	200ul	EUR 420

EDEM1 Antibody
3904-100	Biovision	each	EUR 444

EDEM1 Antibody
3904-30T	Biovision	each	EUR 175.2

EDEM1 Blocking Peptide
33R-5674	Fitzgerald	100 ug	EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of EDEM1 antibody, catalog no. 70R-6871

EDEM1 Blocking Peptide
20-abx161085	Abbexa	EUR 326.40 EUR 493.20	1 mg 5 mg

EDEM1 Polyclonal Antibody
ABP58453-003ml	Abbkine	0.03ml	EUR 189.6
Description: A polyclonal antibody for detection of EDEM1 from Human, Mouse. This EDEM1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EDEM1 protein

EDEM1 Polyclonal Antibody
ABP58453-01ml	Abbkine	0.1ml	EUR 346.8
Description: A polyclonal antibody for detection of EDEM1 from Human, Mouse. This EDEM1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EDEM1 protein

EDEM1 Polyclonal Antibody
ABP58453-02ml	Abbkine	0.2ml	EUR 496.8
Description: A polyclonal antibody for detection of EDEM1 from Human, Mouse. This EDEM1 antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EDEM1 protein

EDEM1 Conjugated Antibody
C47091	SAB	100ul	EUR 476.4

EDEM1 Conjugated Antibody
C45971	SAB	100ul	EUR 476.4

EDEM1 Blocking Peptide
DF9502-BP	Affbiotech	1mg	EUR 234

EDEM1 Polyclonal Antibody
ES9648-100ul	ELK Biotech	100ul	EUR 334.8
Description: A Rabbit Polyclonal antibody against EDEM1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

EDEM1 Polyclonal Antibody
ES9648-50ul	ELK Biotech	50ul	EUR 248.4
Description: A Rabbit Polyclonal antibody against EDEM1 from Human/Mouse. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-EDEM1 antibody
STJ190806	St John's Laboratory	200 µl	EUR 236.4
Description: Unconjugated Rabbit polyclonal to EDEM1

Anti-EDEM1 (2D3)
YF-MA16973	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to EDEM1

EDEM1 Blocking Peptide
3904BP-50	Biovision	each	EUR 183.6

EDEM1 Antibody, HRP conjugated
1-CSB-PA838793LB01HU	Cusabio	EUR 380.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

EDEM1 Antibody, FITC conjugated
1-CSB-PA838793LC01HU	Cusabio	EUR 380.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

EDEM1 Antibody, Biotin conjugated
1-CSB-PA838793LD01HU	Cusabio	EUR 380.40 EUR 402.00	100ul 50ul
Description: A polyclonal antibody against EDEM1. Recognizes EDEM1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Mouse EDEM1 shRNA Plasmid
20-abx980418	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Human EDEM1 shRNA Plasmid
20-abx956466	Abbexa	EUR 961.20 EUR 1345.20	150 µg 300 µg

Mouse Edem1 ELISA KIT
ELI-26124m	Lifescience Market	96 Tests	EUR 1038

Human EDEM1 ELISA KIT
ELI-47111h	Lifescience Market	96 Tests	EUR 988.8

EDEM1 Recombinant Protein (Human)
RP056046	ABM	100 ug	Ask for price

EDEM1 Recombinant Protein (Mouse)
RP130853	ABM	100 ug	Ask for price

EDEM1 ORF Vector (Human) (pORF)
ORF018683	ABM	1.0 ug DNA	EUR 486

Edem1 ORF Vector (Mouse) (pORF)
ORF043619	ABM	1.0 ug DNA	EUR 607.2

Polyclonal EDEM1 antibody - N-terminal region
AMM07002G	Leading Biology	0.05mg	EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human EDEM1 - N-terminal region. This antibody is tested and proven to work in the following applications:

EDEM1 sgRNA CRISPR Lentivector set (Human)
K0653401	ABM	3 x 1.0 ug	EUR 406.8

Edem1 sgRNA CRISPR Lentivector set (Mouse)
K4015601	ABM	3 x 1.0 ug	EUR 406.8

EDEM1 sgRNA CRISPR Lentivector (Human) (Target 1)
K0653402	ABM	1.0 ug DNA	EUR 184.8

EDEM1 sgRNA CRISPR Lentivector (Human) (Target 2)
K0653403	ABM	1.0 ug DNA	EUR 184.8

EDEM1 sgRNA CRISPR Lentivector (Human) (Target 3)
K0653404	ABM	1.0 ug DNA	EUR 184.8

Edem1 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4015602	ABM	1.0 ug DNA	EUR 184.8

Edem1 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4015603	ABM	1.0 ug DNA	EUR 184.8

Edem1 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4015604	ABM	1.0 ug DNA	EUR 184.8

EDEM1 Protein Vector (Human) (pPB-C-His)
PV074729	ABM	500 ng	EUR 662.4

EDEM1 Protein Vector (Human) (pPB-N-His)
PV074730	ABM	500 ng	EUR 662.4

EDEM1 Protein Vector (Human) (pPM-C-HA)
PV074731	ABM	500 ng	EUR 662.4

EDEM1 Protein Vector (Human) (pPM-C-His)
PV074732	ABM	500 ng	EUR 662.4

EDEM1 Protein Vector (Mouse) (pPB-C-His)
PV174474	ABM	500 ng	EUR 723.6

EDEM1 Protein Vector (Mouse) (pPB-N-His)
PV174475	ABM	500 ng	EUR 723.6

EDEM1 Protein Vector (Mouse) (pPM-C-HA)
PV174476	ABM	500 ng	EUR 723.6

EDEM1 Protein Vector (Mouse) (pPM-C-His)
PV174477	ABM	500 ng	EUR 723.6

EDEM1 3'UTR Luciferase Stable Cell Line
TU006559	ABM	1.0 ml	EUR 2799.6

Edem1 3'UTR GFP Stable Cell Line
TU253776	ABM	1.0 ml	Ask for price

Edem1 3'UTR Luciferase Stable Cell Line
TU105597	ABM	1.0 ml	Ask for price

EDEM1 3'UTR GFP Stable Cell Line
TU056559	ABM	1.0 ml	EUR 2799.6

Edem1 3'UTR Luciferase Stable Cell Line
TU203776	ABM	1.0 ml	Ask for price

Edem1 3'UTR GFP Stable Cell Line
TU155597	ABM	1.0 ml	Ask for price

ER Degradation-Enhancing Alpha-Mannosidase-Like Protein 1 (EDEM1) Antibody
20-abx121085	Abbexa	EUR 360.00 EUR 526.80 EUR 226.80	100 ul 200 ul 30 ul

ER Degradation-Enhancing Alpha-Mannosidase-Like Protein 1 (EDEM1) Antibody
20-abx215066	Abbexa	EUR 510.00 EUR 410.40	100 ug 50 ug

EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K0653405	ABM	3 x 1.0 ug	EUR 451.2

Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4015605	ABM	3 x 1.0 ug	EUR 451.2

EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K0653406	ABM	1.0 ug DNA	EUR 200.4

EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K0653407	ABM	1.0 ug DNA	EUR 200.4

EDEM1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K0653408	ABM	1.0 ug DNA	EUR 200.4

Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4015606	ABM	1.0 ug DNA	EUR 200.4

Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4015607	ABM	1.0 ug DNA	EUR 200.4

Edem1 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4015608	ABM	1.0 ug DNA	EUR 200.4

Herein, we report a easy, cheap, and ultrasensitive DNA probe based mostly LFB with CRISPR/Cas and loop-mediated Isothermal Amplification (particularly CIA). The idea behind this strategy is a non-detectable take a look at line on the LFB when the Cas effector protein collaterally cleaves the cognate goal and an ssDNA reporter sequence. The CIA based mostly LFB can detect as little as a single copy cloned Pseudomonas aeruginosa acyltransferase gene, 1 cfu/ml plasmid containing E. coli DH5α pure cultures, in addition to scientific samples without DNA extraction/purification or superior apparatuses. No cross-reactivity with different non-target micro organism was noticed. The bare eye end result readout was obtained in 15 min of LAMP amplification, 30 min of Cas12 response, and 5 min of LFB readout. This platform is powerful and of low price for on-site testing.