Streptococcus pneumoniae is the causative agent of many illnesses, most notably pneumonia. Most of the at current used vaccines to protect in opposition to this pathogen make use of pneumococcal capsular polysaccharides (CPSs) as antigens, nonetheless purifying CPS of ample prime quality has been troublesome.
A purification course of for CPS comprising customary methods resembling ultrafiltration, CTAB precipitation, and chromatography was beforehand established; nonetheless, this approach resulted in extreme cell wall polysaccharide (CWPS) contamination, notably for serotype 5. Thus, a better purification approach that yields CPS of a greater prime quality is required for vaccine progress.
In this study, we significantly diminished CWPS contamination in serotype 5 CPS by bettering the ultrafiltration and CTAB precipitation steps. Moreover, by making use of an acid precipitation course of to further take away totally different impurities, serotype 5 CPS was obtained with a lower impurity resembling decreased nucleic acid contamination. This improved technique was moreover effectively utilized to 14 totally different serotypes (1, 3, 4, 6A, 6B, 7F, 9V, 11A, 14, 18C, 19A, 19F, 22F, and 23F).
To assess the immunogenicity of the CPS from the 15 serotypes, two items of 15-valent pneumococcal conjugate vaccines have been prepared using the sooner purification approach and the improved approach developed proper right here; these vaccines have been administered to a rabbit model.
Enzyme-linked immunosorbent assay and opsonophagocytic assay demonstrated bigger immunogenicity of the conjugate vaccine prepared using CPS produced by the improved purification course of.
[Management of corona virus disease-19 (COVID-19): the Zhejiang experience].
The current epidemic state of affairs of corona virus disease-19 (COVID-19) nonetheless remained excessive. As the National Clinical Research Center for Infectious Diseases, the First Affiliated Hospital of Zhejiang University School of Medicine is the primary medical care center for COVID-19 inZhejiang Province. Based on the present educated consensus carried out by National Health Commission and National Administration of Traditional Chinese Medicine, our workforce summarized and established an environment friendly treatment approach centered on “Four-Anti and Two-Balance” for medical comply with.
The “Four-Anti and Two-Balance”approach included antivirus, anti-shock, anti-hyoxemia, anti-secondary an an infection, and sustaining of water, electrolyte and acid base stability and microecological stability. Meanwhile, built-in multidisciplinarypersonalized treatment was actually useful to reinforce therapeutic impression. The significance of early viralogical detection, dynamic monitoring of inflammatory indexes and chest radiograph was emphasised in medical decision-making.
ECL Western Blotting Substrate |
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KF005 | Affbiotech | 100ml | EUR 168 |
ECL Western Blotting Substrate |
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KF8005 | Affinity Biosciences | 100ml | EUR 140 |
Description: N/A |
ECL Western Blotting Substrate |
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KF8005-100ml | Affinity Biosciences | 100ml | EUR 140 |
ECL Western Blotting Substrate Kit |
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42R-1001 | Fitzgerald | 500 ml | EUR 152 |
Description: ECL Western Blotting Substrate Kit for use in the research laboratory |
ECL Western Blotting Substrate Kit |
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GWB-AXR377 | GenWay Biotech | 50 ml | Ask for price |
ECL Western Blotting Substrate Kit |
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GWB-AXR378 | GenWay Biotech | 500 ml | Ask for price |
ECL Western Blotting Substrate Kit |
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K820-50 | Biovision | each | EUR 183.6 |
ECL Western Blotting Substrate Kit |
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K820-500 | Biovision | each | EUR 405.6 |
ECL Western Blotting Substrate Kit |
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K2187-50 | ApexBio | 50 assays | EUR 173 |
ECL Western Blotting Substrate Kit |
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K2187-500 | ApexBio | 500 assays | EUR 437 |
NuGlo (luminol) ECL Substrate Kit for Western blot, 250 mls |
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80215-250 | Alpha Diagnostics | 1 kit | EUR 196.8 |
Ecl Plus Western Blotting - PK3 |
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RPN2236 | Scientific Laboratory Supplies | PK3 | EUR 710.1 |
NuGlo (luminol) ECL Substrate Kit for Western blot, 50 mls |
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80215-250-S | Alpha Diagnostics | 1 kit | EUR 169.2 |
ECL Plus Western Blotting Substrate |
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AR1196 | BosterBio | 50mL(sufficient reagents for 500 cm2 of membrane) | EUR 121.2 |
ECL Prime Western Blotting Det - EACH |
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RPN2232 | Scientific Laboratory Supplies | EACH | EUR 345.6 |
ECL PRIME WESTERN BLOTTING SYSTEM - EACH |
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GERPN2232 | Scientific Laboratory Supplies | EACH | EUR 329.01 |
Super ECL Plus Western Blotting Substrate |
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EZWB01-SEP100 | Shanghai WSHT Biotechnology | 2x100mL | EUR 72 |
Super ECL Plus Western Blotting Substrate |
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EZWB01-SEP50 | Shanghai WSHT Biotechnology | 2x50mL | EUR 43.2 |
Super ECL Plus Western Blotting Substrate |
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EZWB01-SEP500 | Shanghai WSHT Biotechnology | 2x500mL | EUR 252 |
Amersham ECL DualVue Western Blotting Markers - EACH |
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RPN810 | Scientific Laboratory Supplies | EACH | EUR 298.35 |
Amersham ECL Plex Western Blotting Combination Pack Cy3 Cy5 Hybond ECL - 1KIT |
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RPN998 | Scientific Laboratory Supplies | 1KIT | EUR 342.9 |
Western Blot Kit |
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20-abx098123 | Abbexa |
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Western Blot Kit |
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abx098123-100l | Abbexa | 100 µl | EUR 325 |
Western Blot Kit |
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abx098123-1ml | Abbexa | 1 ml | Ask for price |
Western Blot Kit |
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abx098123-200l | Abbexa | 200 µl | EUR 400 |
Western Blot Blocker |
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20960007-1 | Glycomatrix | 250 g | EUR 32.46 |
Western Blot Blocker |
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20960007-2 | Glycomatrix | 500 g | EUR 60.54 |
Western Blot Marker |
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TTOPP-2301 | Cyagen | 20Lanes | EUR 310.44 |
Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane - EACH |
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RPN2106 | Scientific Laboratory Supplies | EACH | EUR 421.2 |
Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane - EACH |
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RPN2109 | Scientific Laboratory Supplies | EACH | EUR 211.95 |
Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane - 1KIT |
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RPN2134 | Scientific Laboratory Supplies | 1KIT | EUR 643.95 |
Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane - EACH |
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RPN2209 | Scientific Laboratory Supplies | EACH | EUR 328.05 |
Amersham ECL Western Blotting Systemor detection for 1000 cm2 membrane - EACH |
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RPN2108 | Scientific Laboratory Supplies | EACH | EUR 367.2 |
SSB Western Blot Kit |
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GWB-4759BD | GenWay Biotech | Kit | Ask for price |
ERG Western Blot Kit |
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GWB-589385 | GenWay Biotech | Kit | Ask for price |
ID4 Western Blot Kit |
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GWB-232633 | GenWay Biotech | Kit | Ask for price |
FST Western Blot Kit |
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GWB-31B3A5 | GenWay Biotech | Kit | Ask for price |
EED Western Blot Kit |
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GWB-31EF96 | GenWay Biotech | Kit | Ask for price |
AHR Western Blot Kit |
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GWB-EFD465 | GenWay Biotech | Kit | Ask for price |
OTC Western Blot Kit |
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GWB-CED6C6 | GenWay Biotech | Kit | Ask for price |
ENG Western Blot Kit |
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GWB-C2F17D | GenWay Biotech | Kit | Ask for price |
Super Western™ ECL Substrate |
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79572-1 | BPS Bioscience | 200 ml | EUR 115 |
Description: This substrate is ideal for quick, sensitive chemiluminescent detection of horseradish peroxidase (HRP). It’s particularly useful for detecting HRP-labeled secondary antibodies or streptavidin-HRP in immunoblotting applications. |
Super Western™ ECL Substrate |
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79572-2 | BPS Bioscience | 500 ml | EUR 195 |
Description: This substrate is ideal for quick, sensitive chemiluminescent detection of horseradish peroxidase (HRP). It’s particularly useful for detecting HRP-labeled secondary antibodies or streptavidin-HRP in immunoblotting applications. |
Super Western™ ECL Substrate |
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79572-3 | BPS Bioscience | 1 L_x000D__x000D_ | EUR 330 |
Description: This substrate is ideal for quick, sensitive chemiluminescent detection of horseradish peroxidase (HRP). It’s particularly useful for detecting HRP-labeled secondary antibodies or streptavidin-HRP in immunoblotting applications. |
ASPN Western Blot Kit |
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GWB-3F3BF8 | GenWay Biotech | Kit | Ask for price |
SPP1 Western Blot Kit |
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GWB-4172CB | GenWay Biotech | Kit | Ask for price |
ETS1 Western Blot Kit |
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GWB-419F49 | GenWay Biotech | Kit | Ask for price |
G3BP Western Blot Kit |
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GWB-46B393 | GenWay Biotech | Kit | Ask for price |
BRD7 Western Blot Kit |
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GWB-51BDA0 | GenWay Biotech | Kit | Ask for price |
ZBP1 Western Blot Kit |
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GWB-5E94FB | GenWay Biotech | Kit | Ask for price |
MSI2 Western Blot Kit |
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GWB-24AE67 | GenWay Biotech | Kit | Ask for price |
GLI2 Western Blot Kit |
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GWB-2A323E | GenWay Biotech | Kit | Ask for price |
TSHR Western Blot Kit |
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GWB-2D5460 | GenWay Biotech | Kit | Ask for price |
RNF2 Western Blot Kit |
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GWB-31E776 | GenWay Biotech | Kit | Ask for price |
ILF2 Western Blot Kit |
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GWB-1149E0 | GenWay Biotech | Kit | Ask for price |
LHX6 Western Blot Kit |
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GWB-13F4C0 | GenWay Biotech | Kit | Ask for price |
TPM1 Western Blot Kit |
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GWB-16A472 | GenWay Biotech | Kit | Ask for price |
GLI1 Western Blot Kit |
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GWB-888E77 | GenWay Biotech | Kit | Ask for price |
SOX5 Western Blot Kit |
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GWB-908D84 | GenWay Biotech | Kit | Ask for price |
NPM1 Western Blot Kit |
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GWB-AA50AA | GenWay Biotech | Kit | Ask for price |
A1BG Western Blot Kit |
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GWB-FA7CD7 | GenWay Biotech | Kit | Ask for price |
LIG4 Western Blot Kit |
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GWB-FAE800 | GenWay Biotech | Kit | Ask for price |
PAX7 Western Blot Kit |
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GWB-DF90D0 | GenWay Biotech | Kit | Ask for price |
PAX7 Western Blot Kit |
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GWB-EEA80D | GenWay Biotech | Kit | Ask for price |
RORA Western Blot Kit |
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GWB-DC713B | GenWay Biotech | Kit | Ask for price |
PDCD4 Western Blot Kit |
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GWB-37F683 | GenWay Biotech | Kit | Ask for price |
TNRC4 Western Blot Kit |
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GWB-3AECE0 | GenWay Biotech | Kit | Ask for price |
GNB1L Western Blot Kit |
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GWB-3C89D6 | GenWay Biotech | Kit | Ask for price |
PITX2 Western Blot Kit |
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GWB-3D38F8 | GenWay Biotech | Kit | Ask for price |
RPS16 Western Blot Kit |
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GWB-407949 | GenWay Biotech | Kit | Ask for price |
STK11 Western Blot Kit |
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GWB-4A089E | GenWay Biotech | Kit | Ask for price |
PTPN2 Western Blot Kit |
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GWB-4A1E76 | GenWay Biotech | Kit | Ask for price |
IDH3A Western Blot Kit |
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GWB-5ED2E6 | GenWay Biotech | Kit | Ask for price |
STAU1 Western Blot Kit |
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GWB-236BD7 | GenWay Biotech | Kit | Ask for price |
GABRP Western Blot Kit |
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GWB-24C55E | GenWay Biotech | Kit | Ask for price |
TRPM3 Western Blot Kit |
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GWB-28C4BA | GenWay Biotech | Kit | Ask for price |
HIPK2 Western Blot Kit |
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GWB-2CAB70 | GenWay Biotech | Kit | Ask for price |
ESRRG Western Blot Kit |
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GWB-306860 | GenWay Biotech | Kit | Ask for price |
WNT2B Western Blot Kit |
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GWB-31DC35 | GenWay Biotech | Kit | Ask for price |
ANXA1 Western Blot Kit |
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GWB-11A960 | GenWay Biotech | Kit | Ask for price |
SATB1 Western Blot Kit |
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GWB-1505B2 | GenWay Biotech | Kit | Ask for price |
RSAD2 Western Blot Kit |
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GWB-158EF5 | GenWay Biotech | Kit | Ask for price |
HTR1A Western Blot Kit |
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GWB-843597 | GenWay Biotech | Kit | Ask for price |
P2RX7 Western Blot Kit |
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GWB-8C5BF1 | GenWay Biotech | Kit | Ask for price |
KCNN2 Western Blot Kit |
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GWB-8E971E | GenWay Biotech | Kit | Ask for price |
SOX10 Western Blot Kit |
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GWB-93F38B | GenWay Biotech | Kit | Ask for price |
ZFP67 Western Blot Kit |
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GWB-9EC907 | GenWay Biotech | Kit | Ask for price |
OLIG2 Western Blot Kit |
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GWB-6EC267 | GenWay Biotech | Kit | Ask for price |
PCBP1 Western Blot Kit |
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GWB-7D2B23 | GenWay Biotech | Kit | Ask for price |
PANX2 Western Blot Kit |
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GWB-F284D9 | GenWay Biotech | Kit | Ask for price |
CLCN3 Western Blot Kit |
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GWB-F33E4A | GenWay Biotech | Kit | Ask for price |
TGFBI Western Blot Kit |
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GWB-F84989 | GenWay Biotech | Kit | Ask for price |
PANX1 Western Blot Kit |
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GWB-FD36EC | GenWay Biotech | Kit | Ask for price |
RUNX2 Western Blot Kit |
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GWB-FFA7E7 | GenWay Biotech | Kit | Ask for price |
ZFP36 Western Blot Kit |
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GWB-B1BCFA | GenWay Biotech | Kit | Ask for price |
PBEF1 Western Blot Kit |
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GWB-EA0DE7 | GenWay Biotech | Kit | Ask for price |
RAB14 Western Blot Kit |
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GWB-EF804C | GenWay Biotech | Kit | Ask for price |
ZFP42 Western Blot Kit |
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GWB-CABDE0 | GenWay Biotech | Kit | Ask for price |
KEAP1 Western Blot Kit |
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GWB-CD9C1D | GenWay Biotech | Kit | Ask for price |
ATP7A Western Blot Kit |
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GWB-D12842 | GenWay Biotech | Kit | Ask for price |
JMJD3 Western Blot Kit |
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GWB-D8EFB3 | GenWay Biotech | Kit | Ask for price |
CLIC1 Western Blot Kit |
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GWB-DA38C2 | GenWay Biotech | Kit | Ask for price |
ARIH2 Western Blot Kit |
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GWB-DB4558 | GenWay Biotech | Kit | Ask for price |
KRT13 Western Blot Kit |
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GWB-C3423B | GenWay Biotech | Kit | Ask for price |
TARDBP Western Blot Kit |
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GWB-3E00CF | GenWay Biotech | Kit | Ask for price |
HNRPA3 Western Blot Kit |
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GWB-1EAFD5 | GenWay Biotech | Kit | Ask for price |
SNRP70 Western Blot Kit |
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GWB-12C365 | GenWay Biotech | Kit | Ask for price |
TWIST1 Western Blot Kit |
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GWB-172C7E | GenWay Biotech | Kit | Ask for price |
HNRPLL Western Blot Kit |
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GWB-72079F | GenWay Biotech | Kit | Ask for price |
SLC1A5 Western Blot Kit |
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GWB-F244A5 | GenWay Biotech | Kit | Ask for price |
TARDBP Western Blot Kit |
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GWB-DC48CE | GenWay Biotech | Kit | Ask for price |
RHOBTB1 Western Blot Kit |
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GWB-16F5AC | GenWay Biotech | Kit | Ask for price |
SLC22A7 Western Blot Kit |
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GWB-A3D4FB | GenWay Biotech | Kit | Ask for price |
SLC12A1 Western Blot Kit |
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GWB-F10DDC | GenWay Biotech | Kit | Ask for price |
ONECUT2 Western Blot Kit |
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GWB-D5E58E | GenWay Biotech | Kit | Ask for price |
SLC22A1 Western Blot Kit |
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GWB-D6EAC0 | GenWay Biotech | Kit | Ask for price |
CEACAM6 Western Blot Kit |
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GWB-DA1310 | GenWay Biotech | Kit | Ask for price |
Rat Placenta Western Blot |
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RW-413 | Zyagen | 1 Blot | EUR 668 |
Western Blot Enhancing Kit |
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K3172120 | Biochain | 1200 cm2 | EUR 123 |
Western Blot Enhancing Kit |
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K3172250 | Biochain | 2500 cm2 | EUR 182 |
Western Blot Detection Kit |
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E-IR-R304A-50Assays | Elabscience Biotech | 50Assays | EUR 210 |
Western Blot Detection Kit |
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E-IR-R304A-each | Elabscience Biotech | each | Ask for price |
cyclin G2 Western Blot Kit |
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GWB-127000 | GenWay Biotech | Kit | Ask for price |
Amersham ECL Plex Western Blotting Combination Pack Cy3 Cy5 HybondLFP - EACH |
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RPN999 | Scientific Laboratory Supplies | EACH | EUR 315.9 |
Skin Western Blot, 14 species |
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AW-101 | Zyagen | 1 Blot | EUR 1031 |
Lung Western Blot, 14 species |
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AW-601 | Zyagen | 1 Blot | EUR 1031 |
Brain Western Blot, 14 species |
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AW-201 | Zyagen | 1 Blot | EUR 1031 |
Colon Western Blot, 14 species |
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AW-311 | Zyagen | 1 Blot | EUR 1031 |
Liver Western Blot, 14 species |
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AW-314 | Zyagen | 1 Blot | EUR 1031 |
Ovary Western Blot, 14 species |
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AW-406 | Zyagen | 1 Blot | EUR 1031 |
Heart Western Blot, 14 species |
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AW-801 | Zyagen | 1 Blot | EUR 1031 |
Rat Whole Embryo Western Blot |
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RW-104 | Zyagen | 1 Blot | EUR 668 |
Testis Western Blot, 14 species |
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AW-401 | Zyagen | 1 Blot | EUR 1031 |
Kidney Western Blot, 14 species |
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AW-901 | Zyagen | 1 Blot | EUR 1031 |
AHR Western Blot kit (AWBK32243) |
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AWBK32243 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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ENG Western Blot kit (AWBK33068) |
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AWBK33068 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
ID4 Western Blot kit (AWBK38058) |
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AWBK38058 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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EED Western Blot kit (AWBK38384) |
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AWBK38384 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
SSB Western Blot kit (AWBK40462) |
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AWBK40462 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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OTC Western Blot kit (AWBK41766) |
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AWBK41766 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
FST Western Blot kit (AWBK42287) |
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AWBK42287 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
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TMB Substrate, for Western Blot |
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F100029 | Cygnus Technologies | 1000 ml | EUR 992.4 |
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
TMB Substrate, for Western Blot |
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F129-100 | Cygnus Technologies | 100 ml | EUR 298.8 |
Description: TMB Substrate, for Western Blot by Cygnus Technologies is available in Europe via Gentaur. |
Rat Mammary Gland Western Blot |
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RW-414 | Zyagen | 1 Blot | EUR 668 |
Augurin (Human) - Western Blot Kit |
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WBK-012-25 | PHOENIX PEPTIDE | 1 kit | EUR 822.96 |
Ghrelin (Human) - Western Blot Kit |
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WBK-031-30 | PHOENIX PEPTIDE | 1 kit | EUR 822.96 |
Dog Tissue Premade Western Blot |
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DW-MT-1 | Zyagen | 1 Blot | EUR 1031 |
Muscles Western Blot, 14 species |
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AW-102 | Zyagen | 1 Blot | EUR 1031 |
Stomach Western Blot, 14 species |
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AW-302 | Zyagen | 1 Blot | EUR 1031 |
ERG Western Blot kit (AWBK100625) |
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AWBK100625 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
GLI2 Western Blot kit (AWBK31885) |
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AWBK31885 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
PAX4 Western Blot kit (AWBK32064) |
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AWBK32064 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
GLI1 Western Blot kit (AWBK32368) |
|||
AWBK32368 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
PAX7 Western Blot kit (AWBK32393) |
|||
AWBK32393 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
LHX6 Western Blot kit (AWBK32553) |
|||
AWBK32553 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
PAX7 Western Blot kit (AWBK32742) |
|||
AWBK32742 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
SOX5 Western Blot kit (AWBK33323) |
|||
AWBK33323 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
A1BG Western Blot kit (AWBK33810) |
|||
AWBK33810 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
NPM1 Western Blot kit (AWBK34094) |
|||
AWBK34094 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
LIG4 Western Blot kit (AWBK34122) |
|||
AWBK34122 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
RNF2 Western Blot kit (AWBK34290) |
|||
AWBK34290 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
ILF2 Western Blot kit (AWBK35731) |
|||
AWBK35731 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
SPP1 Western Blot kit (AWBK36677) |
|||
AWBK36677 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
ZBP1 Western Blot kit (AWBK36711) |
|||
AWBK36711 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
G3BP Western Blot kit (AWBK37713) |
|||
AWBK37713 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
BRD7 Western Blot kit (AWBK39018) |
|||
AWBK39018 | Aviva Systems Biology | 10 reactions | EUR 776.4 |
Description:
|
Sputum was observed with the easiest optimistic charge of RT-PCR outcomes. Viral nucleic acids might very effectively be detected in10% victims’blood samples at acute periodand 50% of victims had optimistic RT-PCR outcomes in their feces. We moreover isolated alive viral strains from feces, indicating potential infectiousness of feces.Dynamic cytokine detection was important to effectively timed identifyingcytokine storms and utility of artificial liver blood purification system.
The “Four-Anti and Two-Balance”strategyeffectively elevated remedy charge and diminished mortality. Early antiviral treatment might alleviate sickness severity and forestall illness improvement, and we found lopinavir/ritonavir blended with abidol confirmed antiviraleffects in COVID-19. Shock and hypoxemia have been usually caused by cytokine storms.